Human PI 3-Kinase p110 delta Antibody

Name: Human PI 3-Kinase p110 delta Antibody
Brand: R&D Systems
SKU: MAB2687
Rating: 4.3 (4 reviews)

Catalog #: MAB2687
2 Citations 4 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2687
MAB2687-SP

Detection of Human PI 3-Kinase p110δ by Western Blot.

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All PI 3-Kinase p110 delta Antibodies

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Citations (2)

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Reviews (4)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PI 3-Kinase p110 delta Antibody Summary

Species Reactivity

Human

Specificity

Detects endogenous human PI 3-kinase p110δ in Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 275144

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human PI 3-Kinase p110δ
Cys391-Phe542
Accession # O00329

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human PI 3-Kinase p110δ by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Jurkat human acute T cell leukemia cell line, and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human PI 3-Kinase p110d Monoclonal Antibody (Catalog # MAB2687) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PI 3-Kinase p110 delta at approximately 110 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PI 3-Kinase p110 delta

Class IA phosphoinositide 3-kinases (PI 3-kinases) are a family of p85/p110 heterodimeric lipid kinases that function as signal transducers downstream of growth factor receptors, mainly receptor tyrosine kinases. Mammals express three class IA catalytic subunits: p110 alpha, p110 beta, and p110δ. The p110δ subunit is expressed predominantly in leukocytes, indicating that it may play a role in immune signaling.

Long Name

Protein Tyrosine Phosphatase 1D

Entrez Gene IDs

5293 (Human)

Alternate Names

EC 2.7.1; EC 2.7.1.153; p110D; P110DELTA; Phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit delta; phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta isoform; phosphoinositide-3-kinase C; phosphoinositide-3-kinase, catalytic, delta polypeptide; PI 3Kinase p110 delta; PI 3-Kinase p110 delta; PI3K; PI3K-delta; PI3-kinase p110 subunit delta; PI3-kinase subunit delta; PIK3CD; PtdIns-3-kinase subunit delta; PtdIns-3-kinase subunit p110-delta

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human PI 3-Kinase p110 delta Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Identification of HSP90 inhibitors as a novel class of senolytics
Authors: H Fuhrmann-S, YY Ling, J Zhao, SJ McGowan, Y Zhu, RW Brooks, D Grassi, SQ Gregg, JL Stripay, A Dorronsoro, L Corbo, P Tang, C Bukata, N Ring, M Giacca, X Li, T Tchkonia, JL Kirkland, LJ Niedernhof, PD Robbins
Nat Commun, 2017-09-04;8(1):422.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
PI3K? and PI3K? isoforms have distinct functions in regulating pro-tumoural signalling in the multiple myeloma microenvironment
Authors: RE Piddock, N Loughran, CR Marlein, SD Robinson, DR Edwards, S Yu, GE Pillinger, Z Zhou, L Zaitseva, MJ Auger, SA Rushworth, KM Bowles
Blood Cancer J, 2017-03-10;7(3):e539.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot