Human Podocalyxin APC-conjugated Antibody

Catalog #: FAB1658A Datasheet / COA / SDS

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FAB1658A

Detection of Podocalyxin in BG01V Human Embryonic Stem Cells by Flow Cytometry.

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All Podocalyxin Antibodies

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Human Podocalyxin APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Podocalyxin in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse Podocalyxin or recombinant human Endoglycan is observed.

Source

Monoclonal Mouse IgG_2A Clone # 222328

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Podocalyxin
Ser23-Arg427
Accession # AAB61574

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Podocalyxin in BG01V Human Embryonic Stem Cells by Flow Cytometry. BG01V human embryonic stem cells were stained with Mouse Anti-Human Podocalyxin APC-conjugated Monoclonal Antibody (Catalog # FAB1658A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Podocalyxin

Podocalyxin, also known as Podocalyxin-like protein-1 (PCLP1 or PODXL), is a type I transmembrane glycoprotein. It belongs to the CD34/Podocalyxin family of sialomucins that share structural similarity and sequence homology (1). Podocalyxin is a major sialoprotein in the podocytes of the kidney glomerulus and is also expressed by both endothelium and multipotent hematopoietic progenitors (2). It has been identified as a novel cell surface marker for hemangioblasts, the common precursors of hematopoietic and endothelial cells.

References

Li, J. et al. (2001) DNA Seq. 12:407.
Hara, T. et al. (1999) Immunity 11:567.

Entrez Gene IDs

5420 (Human); 27205 (Mouse)

Alternate Names

GCTM; Gp200MGC138240; PCLP; PCLP1; PCLP-1; PCLPGCTM-2 antigen; PCpodocalyxin; POD XL; Podocalyxin; Podocalyxin-like protein 1; podocalyxin-like; PODXL