Human PP2A Catalytic Subunit Antibody

Catalog #: MAB16531 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB16531
MAB16531-SP

All PP2A Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PP2A Catalytic Subunit Antibody Summary

Species Reactivity

Human

Specificity

Detects human PP2A Catalytic Subunit in direct ELISAs. Because PP2A-alpha and PP2A-beta Catalytic Subunits are identical in the region used for the antigen, it is expected that both isoforms will be detected by the antibody.

Source

Monoclonal Rat IgG_2B Clone # 234532

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human PP2A-alpha Catalytic Subunit
Trp209-Leu309
Accession # P67775

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

8-25 µg/mL

Immersion fixed paraffin-embedded sections of human normal liver, normal small intestine, and colon cancer tissue

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PP2A

PP2A, also known as PPP2CA and PP2CA, is a serine/threonine phosphatase.

Entrez Gene IDs

5515 (Human); 51792 (Mouse)

Alternate Names

EC 3.1.3.16; PP2A; PP2A-alpha; PP2Ac; PP2CA; PP2Calpha; PPP2CA; protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform; protein phosphatase 2, catalytic subunit, alpha isozyme; protein phosphatase 2A catalytic subunit, alpha isoform; Replication protein C; RP-C; serine/threonine protein phosphatase 2A, catalytic subunit, alpha isoform; serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform