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Human/Primate IL-17/IL-17A Biotinylated Antibody

Catalog #: BAF317
15 Citations 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
BAF317
IL‑17/IL‑17A in Human Crohn's Disease Intestine.
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Citations (15)
FAQs
Supplemental Products
Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Primate IL-17/IL-17A Biotinylated Antibody Summary

Species Reactivity
Human, Primate
Specificity
Detects human and primate IL-17 in direct ELISAs. In sandwich immunoassays, approximately 22% cross-reactivity with rhIL-17A/F heterodimer is observed, and less than 0.05% cross‑reactivity with recombinant mouse (rm) IL‑17, recombinant human (rh) IL-10, rhIL-12, rhIL-16, rhIFN-gamma, rhIL-17B, rhIL-17C, rhIL-17D, rhIL-17E and rhIL-17F is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human IL-17
Ile20-Ala155
Accession # Q16552
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Immunohistochemistry
0.3-15 µg/mL
See below

Human/Primate IL-17 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Human/Primate IL‑17/IL‑17A Antibody (Catalog # MAB317)

Standard: Recombinant Human IL-17A Protein, CF (Catalog # 317-ILB)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry IL-17/IL-17A antibody in Human Crohn's Disease Intestine by Immunohistochemistry (IHC-P). View Larger

IL‑17/IL‑17A in Human Crohn's Disease Intestine. IL-17/IL-17A was detected in immersion fixed paraffin-embedded sections of human Crohn's disease intestine using Goat Anti-Human/Primate IL-17/IL-17A Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF317) at 0.3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-17/IL-17A

Interleukin 17 (also known as CTLA-8) is a T cell-expressed pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpesvirus Saimiri. cDNA clones encoding IL-17 have been isolated from activated rat, mouse and human T cells. Human IL-17 cDNA encodes a 155 amino acid (aa) residue precursor protein with a 19 amino acid residue signal peptide that is cleaved to yield the 136 aa residue mature IL-17 containing one potential N-linked glycosylation site. Both recombinant and natural IL-17 have been shown to exist as disulfide-linked homodimers. At the amino acid level, human IL-17 shows 72% and 63% sequence identity with herpesvirus and rat IL-17, respectively. An IL-17 specific mouse cell surface receptor (IL-17 R) has been cloned. While the expression of IL-17 mRNA is restricted to activated T cells, the expression of mIL-17 R mRNA has been detected in virtually all cells and tissues tested. IL-17 exhibits multiple biological activities on a variety of cells including the induction of IL-6 and IL-8 production in fibroblasts, the enhancement of surface expression of ICAM-1 in fibroblasts, activation of NF-kappa B and costimulation of T cell proliferation. The non-glycosylated, E. coli-expressed rhIL-17 has been shown to be active on human as well as mouse cell lines.

References
  1. Yao, Z. et al. (1995) J. Immunol. 155:5483.
  2. Yao, Z. et al. (1995) Immunity 3:811.
  3. Rouvier, E. et al. (1993) J. Immunol. 150:5445.
Long Name
Interleukin 17
Entrez Gene IDs
3605 (Human); 16171 (Mouse); 301289 (Rat); 449530 (Porcine); 481837 (Canine); 102119976 (Cynomolgus Monkey)
Alternate Names
CTLA8; CTLA-8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; IL17; IL-17; IL17A; IL-17A; IL-17Acytotoxic T-lymphocyte-associated protein 8; IL-17CTLA-8; IL17interleukin-17A; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A

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Citations for Human/Primate IL-17/IL-17A Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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  1. Hypothermic Ex Situ Perfusion of Human Limbs With Acellular Solution for 24 Hours
    Authors: Valentin Haug, Branislav Kollar, Sotirios Tasigiorgos, Yori Endo, Martin Kauke, Ali-Farid Safi et al.
    Transplantation
  2. Cervico-Vaginal Inflammatory Cytokine and Chemokine Responses to Two Different SIV Immunogens
    Authors: Nikki P. L. Toledo, Hongzhao Li, Robert W. Omange, Tamara G. Dacoba, Jose Crecente-Campo, Dane Schalk et al.
    Frontiers in Immunology
  3. The Global Phosphorylation Landscape of SARS-CoV-2 Infection
    Authors: Mehdi Bouhaddou, Danish Memon, Bjoern Meyer, Kris M. White, Veronica V. Rezelj, Miguel Correa Marrero et al.
    Cell
  4. The vitamin D analogue calcipotriol promotes an anti-tumorigenic phenotype of human pancreatic CAFs but reduces T cell mediated immunity
    Authors: L Gorchs, S Ahmed, C Mayer, A Knauf, C Fernández, M Svensson, R Heuchel, E Rangelova, P Bergman, H Kaipe
    Sci Rep, 2020-10-15;10(1):17444.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Capture
  5. T Cell-Intrinsic IRF5 Regulates T Cell Signaling, Migration, and Differentiation and Promotes Intestinal Inflammation
    Authors: J Yan, SP Pandey, BJ Barnes, JR Turner, C Abraham
    Cell Rep, 2020-06-30;31(13):107820.
    Species: Human
    Sample Types: Whole Cells
    Applications: ELISA Capture
  6. Diverse Human VH antibody fragments with bio-therapeutic properties from the Crescendo Mouse
    Authors: Y Teng, JL Young, B Edwards, P Hayes, L Thompson, C Johnston, C Edwards, Y Sanders, M Writer, D Pinto, Y Zhang, M Roode, P Chovanec, L Matheson, AE Corcoran, A Fernandez, L Montoliu, B Rossi, V Tosato, K Gjuracic, D Nikitin, C Bruschi, B McGuinness, T Sandal, M Romanos
    N Biotechnol, 2019-10-07;55(0):65-76.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: ELISA Detection
  7. Fisetin, a 3,7,3',4'-Tetrahydroxyflavone Inhibits the PI3K/Akt/mTOR and MAPK Pathways and Ameliorates Psoriasis Pathology in 2D and 3D Organotypic Human Inflammatory Skin Models
    Authors: JC Chamcheu, S Esnault, VM Adhami, AL Noll, S Banang-Mbe, T Roy, SS Singh, S Huang, KG Kousoulas, H Mukhtar
    Cells, 2019-09-15;8(9):.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Detection
  8. IL-33 Attenuates Sepsis by Inhibiting IL-17 Receptor Signaling through Upregulation of SOCS3
    Authors: R Lv, J Zhao, M Lei, D Xiao, Y Yu, J Xie
    Cell. Physiol. Biochem., 2017-08-09;42(5):1961-1972.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development (Detection)
  9. Interleukin-10 and prostaglandin E2 have complementary but distinct suppressive effects on Toll-like receptor-mediated dendritic cell activation in ovarian carcinoma
    Authors: E Brencicova, AL Jagger, HG Evans, M Georgouli, A Laios, S Attard Mon, G Mehra, J Spencer, AA Ahmed, S Raju-Kanki, LS Taams, SS Diebold
    PLoS ONE, 2017-04-14;12(4):e0175712.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Detection)
  10. Increase in circulating Th17 cells during anti-TNF therapy is associated with ultrasonographic improvement of synovitis in rheumatoid arthritis
    Authors: Dobrina N Hull
    Arthritis Res. Ther, 2016-12-23;18(1):303.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Detection)
  11. IL-10 suppresses Th17 cells and promotes regulatory T cells in the CD4+ T cell population of rheumatoid arthritis patients.
    Authors: Heo YJ, Joo YB, Oh HJ, Park MK, Heo YM, Cho ML, Kwok SK, Ju JH, Park KS, Cho SG, Park SH, Kim HY, Min JK
    Immunol. Lett., 2009-11-04;127(2):150-6.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  12. Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays.
    Authors: Pfleger C, Schloot N, ter Veld F
    J. Immunol. Methods, 2007-10-22;329(1):214-8.
    Species: Human
    Sample Types: Serum
    Applications: Luminex Development
  13. Ultrasensitive flow-based immunoassays using single-molecule counting.
    Authors: Todd J, Freese B, Lu A, Held D, Morey J, Livingston R, Goix P
    Clin. Chem., 2007-09-21;53(11):1990-5.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  14. Marked differences in human melanoma antigen-specific T cell responsiveness after vaccination using a functional microarray.
    Authors: Chen DS, Soen Y, Stuge TB
    PLoS Med., 2005-09-20;2(10):e265.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  15. Plasma cell-like morphology of Th1-cytokine-producing cells associated with the loss of CD3 expression.
    Authors: Page G, Sattler A, Kersten S, Thiel A, Radbruch A, Miossec P
    Am. J. Pathol., 2004-02-01;164(2):409-17.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P

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Reviews for Human/Primate IL-17/IL-17A Biotinylated Antibody

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Human/Primate IL-17/IL-17A Biotinylated Antibody
By Anonymous on 08/22/2018
Application: ELISA Sample Tested: Cell culture supernatant Species: Human

The antibody was used as a secondary in an IL17A immunoassay.

ELISA Human/Primate IL-17/IL-17A Biotinylated Antibody BAF317
Human/Primate IL-17/IL-17A Biotinylated Antibody
By Balaji Mahender on 01/18/2018
Application: ELISA Sample Tested: Serum and Plasma Species: Human