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Human/Primate IL-2 Biotinylated Antibody

Catalog #: BAF202
9 Citations 1 Review Datasheet / COA / SDS
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BAF202
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IL‑2 in Human PBMCs.
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Citations (9)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Primate IL-2 Biotinylated Antibody Summary

Species Reactivity
Human, Primate
Specificity
Detects human and primate IL-2 in ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human IL-2
Ala21-Thr153
Accession # P60568
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human IL‑2 (Catalog # 202-IL)
Immunocytochemistry
5-15 µg/mL
See below

Human/Primate IL-2 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Human/Primate IL‑2 Antibody (Catalog # MAB602)

Standard: Recombinant Human IL-2 Protein (Catalog # 202-IL)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry IL‑2 antibody in Human PBMCs by Immunocytochemistry (ICC). View Larger

IL‑2 in Human PBMCs. IL‑2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PMA and ionomycin using 15 µg/mL Goat Anti-Human/Primate IL‑2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF202) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunocytochemistry IL‑2 antibody in Human PBMCs by Immunocytochemistry (ICC). View Larger

IL‑2 in Human PBMCs. IL-2 was detected in immersion fixed PMA-, ionomycin-, and monensin-stimulated human peripheral blood mononuclear cells (PBMCs) using 10 µg/mL Goat Anti-Human/Primate IL-2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF202) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-2

Interleukin-2 (IL-2) is a O-glycosylated, four alpha -helix bundle cytokine that has potent stimulatory activity for antigen-activated T cells. It is expressed by CD4+ and CD8+ T cells, gamma δ T cells, B cells, dendritic cells, and eosinophils (1 - 3). Mature human IL-2 shares 56% and 66% aa sequence identity with mouse and rat IL-2, respectively. Human and mouse IL-2 exhibit cross-species activity (4). The receptor for IL-2 consists of three subunits that are present on the cell surface in varying preformed complexes (5 - 7). The 55 kDa IL-2 R alpha is specific for IL-2 and binds with low affinity. The 75 kDa IL-2 R beta, which is also a component of the IL-15 receptor, binds IL-2 with intermediate affinity. The 64 kDa common gamma chain gamma c/IL-2 R gamma, which is shared with the receptors for IL-4, -7, -9, -15, and -21, does not independently interact with IL-2. Upon ligand binding, signal transduction is performed by both IL-2 R beta and gamma c. IL-2 is best known for its autocrine and paracrine activity on T cells. It drives resting T cells to proliferate and induces IL-2 and IL-2 R alpha synthesis (1, 2). It contributes to T cell homeostasis by promoting the Fas-induced death of naïve CD4+ T cells but not activated CD4+ memory lymphocytes (8). IL-2 plays a central role in the expansion and maintenance of regulatory T cells, although it inhibits the development of Th17 polarized cells (9 - 11). Thus, IL-2 may be a key cytokine in the natural suppression of autoimmunity (12, 13).

References
  1. Ma, A. et al. (2006) Annu. Rev. Immunol. 24:657.
  2. Gaffen, S.L. and K.D. Liu (2004) Cytokine 28:109.
  3. Taniguchi, T. et al. (1983) Nature 302:305.
  4. Mosmann, T.R. et al. (1987) J. Immunol. 138:1813.
  5. Liparoto, S.F. et al. (2002) Biochemistry 41:2543.
  6. Wang, X. et al. (2005) Science 310:1159.
  7. Bodnar, A. et al. (2008) Immunol. Lett. 116:117.
  8. Jaleco, S. et al. (2003) J. Immunol. 171:61.
  9. Malek, T.R. (2003) J. Leukoc. Biol. 74:961.
  10. Laurence, A. et al. (2007) Immunity 26:371.
  11. Kryczek, I. et al. (2007) J. Immunol. 178:6730.
  12. Afzali, B. et al. (2007) Clin. Exp. Immunol. 148:32.
  13. Fehervari, Z. et al. (2006) Trends Immunol. 27:109.
Long Name
Interleukin 2
Entrez Gene IDs
3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit)
Alternate Names
Aldesleukin; IL2; IL-2; IL-2lymphokine; interleukin 2; interleukin-2; involved in regulation of T-cell clonal expansion; Proleukin; T cell growth factor; T-cell growth factor; TCGF

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Citations for Human/Primate IL-2 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Evaluation of Single-Cell Cytokine Secretion and Cell-Cell Interactions with a Hierarchical Loading Microwell Chip
    Authors: Y Zhou, N Shao, R Bessa de C, P Zhang, Y Ma, X Liu, F Huang, RF Wang, L Qin
    Cell Rep, 2020-04-28;31(4):107574.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Capture
  2. Interference of the T Cell and Antigen-Presenting Cell Costimulatory Pathway Using CTLA4-Ig (Abatacept) Prevents Staphylococcal Enterotoxin B Pathology
    Authors: SJ Whitfield, C Taylor, JE Risdall, GD Griffiths, JT Jones, ED Williamson, S Rijpkema, L Saraiva, S Vessillier, AC Green, AJ Carter
    J. Immunol, 2017-03-20;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  3. Key Residues at Third CDR3? Position Impact Structure and Antigen Recognition of Human Invariant NK TCRs
    Authors: Kenji Chamoto
    J. Immunol, 2016-12-21;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: ELISA Development (Detection)
  4. Functional characterization of human T cell hyporesponsiveness induced by CTLA4-Ig.
    Authors: Rochman Y, Yukawa M, Kartashov A, Barski A
    PLoS ONE, 2015-04-10;10(4):e0122198.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  5. Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo.
    Authors: Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB
    AIDS Res. Hum. Retroviruses, 2008-03-01;24(3):453-62.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Luminex Development
  6. Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays.
    Authors: Pfleger C, Schloot N, ter Veld F
    J. Immunol. Methods, 2007-10-22;329(1):214-8.
    Species: Human
    Sample Types: Serum
    Applications: Luminex Development
  7. Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
    Authors: Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM
    Blood, 2007-02-08;109(10):4272-9.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Luminex Development
  8. Th2 cytokine response in Major Depressive Disorder patients before treatment.
    Authors: Kiryushko D, Pavon L, Novitskaya V, Sandoval-Lopez G, Soroka V, Eugenia Hernandez M, Klingelhofer J, Loria F, Lukanidin E, Estrada I, Berezin V, Perez M, Bock E, Moreno J, Avila U, Leff P, Anton B, Heinze G
    J. Neuroimmunol., 2006-02-02;172(1):156-65.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development
  9. Repeated exposure induces periportal fibrosis in Schistosoma mansoni-infected baboons: role of TGF-beta and IL-4.
    Authors: Farah IO, Mola PW, Kariuki TM, Nyindo M, Blanton RE, King CL
    J. Immunol., 2000-05-15;164(10):5337-43.
    Species: Primate - Papio anubis (Olive Baboon)
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development

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Human/Primate IL-2 Biotinylated Antibody
By Anonymous on 09/17/2019
Application: ELISA Sample Tested: Recombinant protein Species: Human
ELISA Human/Primate IL-2 Biotinylated Antibody BAF202