What are the differences between MAB293 and MAB293R?

Name: Human/Primate VEGF Antibody
Brand: R&D Systems
SKU: MAB293R
Price: 479 USD

MAB293 is produced by culturing a hybridoma cell line, whereas MAB293R is produced via recombinant DNA technology, based on the known, proprietary sequence of MAB293. The benefit of recombinant antibodies is the ability to offer a more consistent antibody, with an indefinite supply. As far as research applications, both products will work the same.

Human/Primate VEGF Antibody

Catalog #: MAB293R Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB293R-SP
MAB293R-500
MAB293R-01M
MAB293R-100

Detection of Human VEGF by Western Blot.

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Human/Primate VEGF Antibody Summary

Species Reactivity

Human, Primate

Specificity

Detects human VEGF 165 and human VEGF 121 in direct ELISAs and Western blots. In ELISAs, this antibody shows approximately 10% cross-reactivity with recombinant mouse (rm) VEGF and rrVEGF and no cross-reactivity with rhVEGF-D.

Source

Recombinant Monoclonal Mouse IgG_2B Clone # 26503R

Purification

Protein A or G purified from cell culture supernatant

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human VEGF 165 Ala27-Arg191 Accession # NP_001165097

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Immunohistochemistry

8-25 µg/mL

See below

Human/Primate VEGF Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Human/Primate VEGF₁₆₅ Biotinylated Antibody (Catalog # BAF293)

Standard: Recombinant Human VEGF 165 Protein (Catalog # 293-VE)

Neutralization

Measured by its ability to neutralize VEGF₁₆₅-induced proliferation in HUVEC human umbilical vein endothelial cells. The Neutralization Dose (ND₅₀) is typically 10-60 ng/mL in the presence of 10 ng/mL Recombinant Human VEGF₁₆₅.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human VEGF by Western Blot. Western blot shows lysates of human lung tissue. PVDF membrane was probed with 2 µg/mL of Recombinant Mouse Anti-Human/Primate VEGF Monoclonal Antibody (Catalog # MAB293R) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for VEGF at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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VEGF in Human Liver. VEGF was detected in immersion fixed paraffin-embedded sections of human liver using Recombinant Mouse Anti-Human/Primate VEGF Monoclonal Antibody (Catalog # MAB293R) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Neutralization

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Cell Proliferation Induced by VEGF₁₆₅and Neutralization by Human VEGF Antibody. Recombinant Human VEGF₁₆₅(Catalog # 293-VE) stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human VEGF₁₆₅(10 ng/mL) is neutralized (green line) by increasing concentrations of Recombinant Mouse Anti-Human/Primate VEGF Monoclonal Antibody (Catalog # MAB293R). The ND₅₀ is typically 10-60 ng/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Background: VEGF

Vascular Endothelial Growth Factor (VEGF or VEGF-A), also known as Vascular Permeability Factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the PDGF family that is characterized by the presence of eight conserved cysteine residues and a cystine knot structure. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189. Human VEGF165 is an approximately 44 kDa molecular weight homodimer sharing 88% aa sequence identity with corresponding regions of mouse and rat, 96% with porcine, 95% with canine, and 93% with feline, equine and bovine VEGF, respectively. VEGF binds the type I transmembrane receptor tyrosine kinases VEGF R1 (also called Flt-1) and VEGF R2 (Flk-1/KDR) on endothelial cells. Although VEGF affinity is highest for binding to VEGF R1, VEGF R2 appears to be the primary mediator of VEGF angiogenic activity. VEGF165 binds the Semaphorin receptor, Neuropilin-1 and promotes complex formation with VEGF R2. VEGF is required during embryogenesis and functions to regulate the proliferation, migration, and survival of endothelial cells. In adults, VEGF functions mainly in wound healing and the female reproductive cycle. Pathologically, it is involved in tumor angiogenesis and vascular leakage. Circulating VEGF levels correlate with disease activity in autoimmune diseases such as rheumatoid arthritis, multiple sclerosis and systemic lupus erythematosus. VEGF is induced by hypoxia and cytokines such as IL-1, IL-6, IL-8, Oncostatin M (OSM) and TNF-alpha.

Long Name

Vascular Endothelial Growth Factor

Entrez Gene IDs

7422 (Human); 22339 (Mouse); 83785 (Rat); 281572 (Bovine); 403802 (Canine); 493845 (Feline); 30682 (Zebrafish)

Alternate Names

MVCD1; VAS; vascular endothelial growth factor A; Vascular permeability factor; Vasculotropin; VEGF; VEGFA; VEGF-A; VEGFMGC70609; VPF; VPFvascular endothelial growth factor

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

FAQs

What are the differences between MAB293 and MAB293R?
- MAB293 is produced by culturing a hybridoma cell line, whereas MAB293R is produced via recombinant DNA technology, based on the known, proprietary sequence of MAB293. The benefit of recombinant antibodies is the ability to offer a more consistent antibody, with an indefinite supply. As far as research applications, both products will work the same.

View all Antibody FAQs