Human PRKD3/nPKC nu Antibody

Catalog #: AF6478 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6478
AF6478-SP

Detection of Human PRKD3/nPKC nu by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PRKD3/nPKC nu Antibody Summary

Species Reactivity

Human

Specificity

Detects human PRKD3/nPKC nu in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human PRKD3/nPKC nu
Ile465-Val559
Accession # O94806

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human PRKD3/nPKC nu by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line and Ramos human Burkitt's lymphoma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human PRKD3/nPKC nu Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6478) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PRKD3/nPKC nu at approximately 105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

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PRKD3/nPKC nu in MCF‑7 Human Cell Line. PRKD3/nPKC nu was detected in immersion fixed MCF-7 human breast cancer cell line using Sheep Anti-Human PRKD3/nPKC nu Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6478) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Sheep IgG Secondary Antibody (green; Catalog # NL012) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PRKD3/nPKC nu

PKC nu (Protein kinase C‑nu; also PKD3 and PRKD3) is a 105‑110 kDa member of the PKD subfamily, CAMK Ser/Thr protein kinase family of enzymes. It is expressed in highly diverse cell types such as pancreatic aciner cells, skeletal muscle cells, B cells and sensory neurons, and it is activated by PKC‑mediated phosphorylation. Active PKC nu associates with membrane compartments, and appears to participate with VAMP2 in cargo transport and vesicular trafficking. It also acts on class II HDACs in the nucleus, resulting in gene transcription. Human PKC nu is 890 amino acids (aa) in length. It contains two DAG‑type zinc finger regions (aa 154‑204 and 271‑321), one PH domain (aa 416‑532) and a large protein kinase domain (aa 576‑832). There are at least 25 utilized Ser/Thr phosphorylation sites that span the length of the molecule. PKC nu possesses one potential splice variant that shows a 17 aa substitution for aa 595‑890. Over aa 465‑559, human PKC nu is 92% aa identical to mouse PKC nu.

Long Name

Protein Kinase D3

Entrez Gene IDs

23683 (Human); 75292 (Mouse); 313834 (Rat)

Alternate Names

EC 2.7.11; EC 2.7.11.13; EPK2; EPK2PKD3; nPKC nu; nPKC-nu; PKC nu; PKC-NU; PRKCN; PRKCNnPKC-NU; PRKD3; Protein kinase C nu type; protein kinase C, nu; protein kinase D3; Protein kinase EPK2; protein-serine/threonine kinase; serine/threonine-protein kinase D3