Human Pro-Collagen I alpha 1 Quantikine ELISA Kit

Catalog #: DPCA00 Datasheet / COA / SDS

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DPCA00

Human Pro-Collagen I alpha 1 ELISA Standard Curve

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human Pro-Collagen I alpha 1 Quantikine ELISA Kit Summary

Sample Type & Volume Required Per Well

Cell Culture Supernates (25 uL), Cell Lysates (25 uL), Tissue Lysates (25 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Saliva (10 uL), Urine (25 uL)

Sensitivity

8.75 pg/mL

Assay Range

78.1 - 5,000 pg/mL (Cell Culture Supernates, Cell Lysates, Tissue Lysates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine)

Specificity

Natural and recombinant human Pro-Collagen I alpha 1.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human Pro-Collagen I alpha 1/COLIA1 (Pro-Collagen I alpha 1) Immunoassay is a 3.5 hour solid-phase ELISA designed to measure human Pro-Collagen I alpha 1 in cell culture supernates, cell lysates, tissue extracts, serum, plasma, saliva, and urine. It contains recombinant human Pro-Collagen I alpha 1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Pro-Collagen I alpha 1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Pro-Collagen I alpha 1.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Cell Culture Supernates, Cell Lysates, Tissue Lysates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	442	1410	2669	448	1378	2677
Standard Deviation	17.8	32.4	50.1	28.9	49.8	109
CV%	4	2.3	1.9	6.4	3.6	4.1

Recovery

The recovery of human Pro-Collagen I alpha 1 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Extraction Buffer (n=1)	105	101-110
Lysis Buffer (n=1)	97	94-101
Media (n=4)	107	97-118
Saliva (n=4)	97	87-114
Urine (n=4)	90	79-111

Linearity

To assess the linearity of the assay, samples containing high concentrations of human Pro-Collagen I alpha 1 were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.

Scientific Data

N/A

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Human Pro-Collagen I alpha 1 ELISA Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Collagen I alpha 1

Type I collagen is the most abundant structural protein of connective tissues such as skin, bone and tendon. It is synthesized as a procollagen molecule which is characterized by a 300 nm triple helical domain flanked by globular N- and C-terminal propeptides. The triple helical domain contains Gly-Xaa-Yaa triplets where Xaa and Yaa are frequently proline and hydroxyproline, respectively. The non-helical propeptides are removed by procollagen N- and C-proteinase activities so that the mature triple helices can self-assemble into collagen fibrils that provide tensile strength to tissues. Type I collagen is a heterotrimer that consists of two alpha 1(I) chains and one alpha 2(I) chain, although homotrimers consisting of three identical alpha 1(I) chains have also been described. This recombinant mini pro-alpha 1(I) collagen consists of a shortened alpha 1(I) chain with following domain structure from N- to C-terminus: N-propeptide, N-telopeptide, the 33 most N-terminal Gly-Xaa-Yaa repeats, the 33 most C-terminal Gly-Xaa-Yaa repeats, C-telopeptide and C-propeptide. The preparation contains a mixture of the full-length molecule, pN collagen I (alpha 1) and the C-terminal propeptide. This truncated pro-alpha 1(I) collagen is a substrate for procollagen N-proteinase and procollagen C-proteinase.

Entrez Gene IDs:

1277 (Human)

Alternate Names:

Alpha-1 type I collagen; COL1A1; COL-IA1; Collagen 1 alpha 1; Collagen 1; collagen alpha 1 chain type I; collagen alpha-1(I) chain; Collagen I alpha 1; collagen of skin, tendon and bone, alpha-1 chain; Collagen Type I Alpha 1 Chain; collagen, type I, alpha 1; Collagen1; Collagen-1; EDSARTH1; OI4; pro-alpha-1 collagen type 1; Type I Procollagen Alpha 1 Chain