Home / Collagen I alpha 1 / Human Pro Collagen I  alpha 1 Antibody

Human Pro Collagen I  alpha 1 Antibody

Catalog #: AF6220
12 Citations 1 Review Datasheet / COA / SDS
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AF6220
AF6220-SP
Detection of Human Pro-Collagen I alpha 1 by Western Blot.
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Citations (12)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Pro Collagen I  alpha 1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Pro-Collagen I alpha 1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human COL3A1 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Collagen I
Gln23-Lys277, Gly1094-Leu1464
Accession # P02452
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
IMR‑90 human lung fibroblast cell line
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human N-Pro Collagen I by Western Blot. View Larger

Detection of Human Pro-Collagen I alpha 1 by Western Blot. Western blot shows lysates of human kidney tissue, human skin tissue, and human cartilage tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Pro-Collagen I alpha 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6220) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Pro-Collagen I alpha 1 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot View Larger

Detection of Human Collagen I by Western Blot. Western blot shows lysates of IMR‑90 human lung fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Pro Collagen I  alpha 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6220) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for Collagen I at approximately 170 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Immunocytochemistry Collagen I a1 antibody in IMR-90 Human Cell Line by Immunocytochemistry (ICC). View Larger

Pro-Collagen I alpha 1 in IMR‑90 Human Cell Line. Pro-Collagen I alpha 1 was detected in immersion fixed IMR-90 human lung fibroblast cell line using Sheep Anti-Human Pro-Collagen I alpha 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6220) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunoprecipitation View Larger

Detection of Human N-Pro Collagen I by Immunoprecipitation. Human N-Pro Collagen I was immunoprecipitated from 500 μg of IMR‑90 human lung fibroblast cell line lysates with 5 μg of Sheep Anti-Human N-Pro Collagen I Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6220). The N-Pro Collagen I-antibody complexes were absorbed using Protein A or Protein G. Immunoprecipitated human N-Pro Collagen I was detected by Western blot using 1 μg/mL of Mouse Anti-Human N-Pro Collagen I Monoclonal Antibody (MAB6220) under reducing conditions and using Western Blot Buffer Group 1.

Simple Western View Larger

Detection of Human Collagen I  alpha 1 by Simple WesternTM. Simple Western lane view shows lysates of IMR‑90 human lung fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for Collagen I  alpha 1 at approximately 232 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human Pro Collagen I  alpha 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6220) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Collagen I alpha 1

Type I collagen is the most abundant structural protein of connective tissues such as skin, bone and tendon. It is synthesized as a procollagen molecule which is characterized by a 300 nm triple helical domain flanked by globular N- and C-terminal propeptides (1). The triple helical domain contains Gly-Xaa-Yaa triplets where Xaa and Yaa are frequently proline and hydroxyproline, respectively. The non-helical propeptides are removed by procollagen N- and C-proteinase activities so that the mature triple helices can self-assemble into collagen fibrils that provide tensile strength to tissues (1). Type I collagen is a heterotrimer that consists of two alpha 1(I) chains and one alpha 2(I) chain, although homotrimers consisting of three identical alpha 1(I) chains have also been described (2). This recombinant mini pro-alpha 1(I) collagen consists of a shortened alpha 1(I) chain with following domain structure from N- to C-terminus: N-propeptide, N‑telopeptide, the 33 most N-terminal Gly-Xaa-Yaa repeats, the 33 most C-terminal Gly-Xaa-Yaa repeats, C-telopeptide and C-propeptide. The preparation contains a mixture of the full-length molecule, pN collagen I( alpha 1) and the C-terminal propeptide. This truncated pro-alpha 1(I) collagen is a substrate for procollagen N-proteinase and procollagen C-proteinase.

References
  1. Canty, E.G. et al. (2005) J. Cell Sci. 118:1341.
  2. Han, S. et al. (2008) J. Mol. Biol. 383:122.
Entrez Gene IDs
1277 (Human)
Alternate Names
Alpha-1 type I collagen; COL1A1; COL-IA1; Collagen 1 alpha 1; Collagen 1; collagen alpha 1 chain type I; collagen alpha-1(I) chain; Collagen I alpha 1; collagen of skin, tendon and bone, alpha-1 chain; Collagen Type I Alpha 1 Chain; collagen, type I, alpha 1; Collagen1; Collagen-1; EDSARTH1; OI4; pro-alpha-1 collagen type 1; Type I Procollagen Alpha 1 Chain

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Citations for Human Pro Collagen I  alpha 1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. Mapping of clonal lineages across developmental stages in human neural differentiation
    Authors: Z You, L Wang, H He, Z Wu, X Zhang, S Xue, P Xu, Y Hong, M Xiong, W Wei, Y Chen
    Cell Stem Cell, 2023-03-17;0(0):.
    Species: Human
    Sample Types: Organoid
    Applications: IHC
  2. Mapping of clonal lineages across developmental stages in human neural differentiation
    Authors: Z You, L Wang, H He, Z Wu, X Zhang, S Xue, P Xu, Y Hong, M Xiong, W Wei, Y Chen
    Cell Stem Cell, 2023;0(0):.
    Species: Human
    Sample Types: Organoid
    Applications: IHC
  3. Transcriptomic and Immunohistochemical Analysis of Progressive Keratoconus Reveal Altered WNT10A in Epithelium and Bowman's Layer
    Authors: JW Foster, RN Parikh, J Wang, KS Bower, M Matthaei, S Chakravart, AS Jun, CG Eberhart, US Soiberman
    Investigative Ophthalmology & Visual Science, 2021-05-03;62(6):16.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  4. Suppression of pancreatic ductal adenocarcinoma growth and metastasis by fibrillar collagens produced selectively by tumor cells
    Authors: C Tian, Y Huang, KR Clauser, S Rickelt, AN Lau, SA Carr, MG Vander Hei, RO Hynes
    Nature Communications, 2021-04-20;12(1):2328.
    Species: Human
    Sample Types: Tissue, Tissue Homogenates
    Applications: IHC, Western Blot
  5. TGF-&beta2 Promotes Oxidative Stress in Human Trabecular Meshwork Cells by Selectively Enhancing NADPH Oxidase 4 Expression
    Authors: VR Rao, EB Stubbs
    Investigative Ophthalmology & Visual Science, 2021-04-01;62(4):4.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  6. Autologous transplant therapy alleviates motor and depressive behaviors in parkinsonian monkeys
    Authors: Y Tao, SC Vermilyea, M Zammit, J Lu, M Olsen, JM Metzger, L Yao, Y Chen, S Phillips, JE Holden, V Bondarenko, WF Block, TE Barnhart, N Schultz-Da, K Brunner, H Simmons, BT Christian, ME Emborg, SC Zhang
    Nature Medicine, 2021-03-01;0(0):.
    Species: Primate - Rhesus macaque
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC
  7. Preclinical Efficacy and Safety of a Human Embryonic Stem Cell-Derived Midbrain Dopamine Progenitor Product, MSK-DA01
    Authors: J Piao, S Zabierowsk, BN Dubose, EJ Hill, M Navare, N Claros, S Rosen, K Ramnarine, C Horn, C Fredrickso, K Wong, B Safford, S Kriks, A El Maarouf, U Rutishause, C Henchcliff, Y Wang, I Riviere, S Mann, V Bermudez, S Irion, L Studer, M Tomishima, V Tabar
    Cell Stem Cell, 2021-02-04;28(2):217-229.e7.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. Extracellular histones stimulate collagen expression in vitro and promote liver fibrogenesis in a mouse model via the TLR4-MyD88 signaling pathway
    Authors: Z Wang, ZX Cheng, ST Abrams, ZQ Lin, ED Yates, Q Yu, WP Yu, PS Chen, CH Toh, GZ Wang
    World Journal of Gastroenterology, 2020-12-21;26(47):7513-7527.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Modeling Progressive Fibrosis with Pluripotent Stem Cells Identifies an Anti-fibrotic Small Molecule
    Authors: P Vijayaraj, A Minasyan, A Durra, S Karumbayar, M Mehrabi, CJ Aros, SD Ahadome, DW Shia, K Chung, JM Sandlin, KF Darmawan, KV Bhatt, CC Manze, MK Paul, DC Wilkinson, W Yan, AT Clark, TM Rickabaugh, WD Wallace, TG Graeber, R Damoiseaux, BN Gomperts
    Cell Rep, 2019-12-10;29(11):3488-3505.e9.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  10. Evidence Suggesting a Role of Iron in a Mouse Model of Nephrogenic Systemic Fibrosis.
    Authors: Bose C, Megyesi J, Shah S, Hiatt K, Hall K, Karaduta O, Swaminathan S
    PLoS ONE, 2015-08-25;10(8):e0136563.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  11. Effect of thrombin on human amnion mesenchymal cells, mouse fetal membranes, and preterm birth.
    Authors: Mogami, Haruta, Keller, Patrick, Shi, Haolin, Word, R Ann
    J Biol Chem, 2014-03-20;289(19):13295-307.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  12. PDGFRbeta expression and function in fibroblasts derived from pluripotent cells is linked to DNA demethylation.
    J. Cell. Sci., 2012-02-17;125(0):2276-87.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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Human Collagen I  alpha 1 Antibody
By Anonymous on 10/18/2016
Application: ICC/IF Sample Tested: decellularized primary fibroblast culture Species: Human
Immunocytochemistry/Immunofluorescence Human Collagen I  alpha 1 Antibody AF6220