Human Protein S/PROS1 Antibody

Catalog #: MAB4036
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4036
MAB4036-SP

Detection of Human Protein S/PROS1 by Western Blot.

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Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Protein S/PROS1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Protein S in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross‑react with recombinant mouse Protein S.

Source

Monoclonal Mouse IgG₁ Clone # 391609

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Protein S
Ala42-Trp670
Accession # P07225

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Protein S/PROS1 by Western Blot. Western blot shows human plasma. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Protein S/PROS1 Monoclonal Antibody (Catalog # MAB4036) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Protein S/PROS1 at approximately 80 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Protein S/PROS1

The PROS1 gene encodes Protein S (PS), a ~70 kDa vitamin-K dependent plasma glycoprotein mainly synthesized by human hepatocytes. PS is a non-enzymatic cofactor in the degradation of coagulation factors Va and VIIIa and thus plays an anticoagulant role. Approximately 60% circulates in complex with C4b-binding protein. Free PS is a mixture of monomers and multimers. The 652 aa PS proprotein is heavily modified with 11 carboxyglutamates, one hydroxyaspartate, disulfides and glycosylation. Human Protein S shows 80% aa identity with mouse Protein S.

Entrez Gene IDs

5627 (Human); 19128 (Mouse); 81750 (Rat)

Alternate Names

EC 3.4.21; PROS; PROS1; protein S (alpha); Protein S; protein Sa; PS21; PS22; PS23; PS24; PS25; PSA; vitamin K-dependent plasma protein S; vitamin K-dependent protein S

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human Protein S/PROS1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Alcohol and HIV-Derived Hepatocyte Apoptotic Bodies Induce Hepatic Stellate Cell Activation
Authors: M New-Aaron, RS Dagur, SS Koganti, M Ganesan, W Wang, E Makarov, M Ogunnaike, KK Kharbanda, LY Poluektova, NA Osna
Biology, 2022-07-14;11(7):.
Species: Human
Sample Types: Whole Cells
Applications: ICC
Human Placental Mesenchymal Stem/Stromal cells (pMSCs) inhibit agonist-induced platelet functions reducing atherosclerosis and thrombosis phenotypes
Authors: A Al Subayyi, YS Basmaeil, R Alenzi, T Khatlani
Journal of Cellular and Molecular Medicine, 2021-09-18;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot