Human Protocadherin-8 Antibody

Catalog #: MAB5434 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5434
MAB5434-SP

Protocadherin‑8 in MCF‑7 Human Cell Line.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Protocadherin-8 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Protocadherin-8 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Protocadherin-1, -10, or -15 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 562615

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Protocadherin-8
Lys30-Thr479
Accession # O95206

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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Protocadherin‑8 in MCF‑7 Human Cell Line. Protocadherin-8 was detected in immersion fixed MCF-7 human breast cancer cell line using Human Protocadherin-8 Monoclonal Antibody (Catalog # MAB5434) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Protocadherin-8

Protocadherin 8 (PCDH-8) is a 160 kDa group F member of the protocadherin family of molecules. It is expressed in breast epithelium, the synaptic membranes of neurons, and presumably forms Ca⁺⁺-dependent homophilic complexes. In breast, it is believed to contribute to a stable epithelial architecture, while in brain, it impacts LTP. Mature human PCDH-8 is a type I transmembrane protein that is 1041 amino acids (aa) in length. It contains a 720aa extracellular domain (ECD) (aa 30-749) plus a 300 aa cytoplasmic region. There are six cadherin domains in the ECD (aa 30-723). Multiple splice variants exist. Two show deletions of aa 134-607 and 781-877, respectively, while a third shows a six aa substitution for aa 365-527. Over aa 30-689, human PCDH-8 shares 93% aa identity with mouse PCDH-8.

Entrez Gene IDs

5100 (Human); 18530 (Mouse); 64865 (Rat)

Alternate Names

Arcadlin; PAPC; PCDH8; protocadherin 8; Protocadherin8; Protocadherin-8