Human PRR5 Antibody

Catalog #: AF5309 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5309
AF5309-SP

Detection of Human PRR5 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PRR5 Antibody Summary

Species Reactivity

Human

Specificity

Detects endogenous human PRR5 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human PRR5
Tyr126-Ala259
Accession # P85299

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Immunogen affinity purified

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human PRR5 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Jurkat human acute T cell leukemia cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Human PRR5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5309) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PRR5 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PRR5

PRR5 (Pro-rich protein 5; also Protor-1) is a 42‑46 kDa member of the Protor family of proteins. It is widely expressed, and forms part of mTORC2, an enzyme complex that regulates Atk and PKC alpha. At present, it appears PRR5 promotes mTORC2 activity. Human PRR5 is 388 amino acids (aa) in length. It possesses two RICTOR-interaction sites (aa 10‑95 and 188‑218) and a C-terminal Pro-rich region. There are multiple splice forms of PRR5. Either individually, or in-common, there is an alternate start site at Met10 ( beta isoform) and Met96 ( gamma isoform), a deletion of aa 45‑71, 161‑388, and 236‑388, a 17 aa insertion after Gly108, and a six aa substitution for aa 1‑107. Over aa 26‑259 of the 41 kDa beta isoform (NP_056181), there is 95% aa identity between human and mouse PRR5.

Long Name

Proline Rich 5 [Renal]

Entrez Gene IDs

55615 (Human); 109270 (Mouse); 315189 (Rat)

Alternate Names

FLJ20185; FLJ20185k; PP610; proline rich 5 (renal); protein observed with Rictor-1; Protor-1; PRR5