Human PTH1R/PTHR1 Biotinylated Antibody

Catalog #: BAF5709 Datasheet / COA / SDS

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BAF5709

All PTH1R/PTHR1 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PTH1R/PTHR1 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human PTH1R/PTHR1 in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human PTH1R/PTHR1
Tyr23-Met189
Accession # Q03431

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human PTH1R/PTHR1

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PTH1R/PTHR1

PTH1 R (Parathyroid hormone receptor # 1; also PTH/PTHr receptor) is a predicted 66‑68 kDa member of the G-protein coupled receptor family # 2. It is expressed on a variety of cell types, including hepatocytes, renal epithelium, smooth muscle cells, and osteoblasts plus chondrocytes. PTH1 R is a receptor for both PTH and PTHrP, and PTH binding promotes Ca⁺⁺-release from bone, mediated by osteoclast formation. Mature human PTH1 R is a 7-transmembrane glycoprotein 567 amino acids (aa) in length. It contains a long, ligand-binding N‑terminal extracellular region (aa 27‑188) and a 130 aa cytoplasmic C-terminal domain. Single aa changes such as Gly121Glu, Ala122Thr and Arg255His can impair PTH1 R signaling. Over aa 1‑189, human PTH1 R shares 88% aa identity with mouse PTH1 R.

Long Name

Parathyroid Hormone Receptor 1

Entrez Gene IDs

5745 (Human); 19228 (Mouse); 56813 (Rat)

Alternate Names

MGC138426; parathyroid hormone 1 receptorMGC138452; parathyroid hormone/parathyroid hormone-related peptide receptor; parathyroid hormone/parathyroid hormone-related protein receptor; PFE; PTH/PTHr receptor; PTH/PTHrP type I receptor; PTH1 receptor; PTH1R; PTHR; PTHR1; PTHRPTHR1parathyroid hormone receptor 1; seven transmembrane helix receptor