Human PTP1B Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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PTP1B in Human Breast Cancer Tissue. PTP1B was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Rabbit Anti-Human PTP1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1366) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Specific labeling was localized to the perinuclear region in glandular epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Detection of Human PTP1B by Western Blot. Western blot shows lysates of TF-1 human erythroleukemic cell line and CHP-100 human neuroblastoma cell line. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human PTP1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1366) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for PTP1B at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Human PTP1B by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for PTP1B at approximately 57 kDa (as indicated) using 10 µg/mL of Rabbit Anti-Human PTP1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1366). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PTP1B
Protein tyrosine phosphatase 1B (PTP1B) is an enzyme that removes phosphate groups covalently attached to tyrosine residues in proteins. This ubiquitously expressed enzyme is anchored in the endoplasmic reticulum by its C-terminal end and has its catalytic regions exposed to the cytosol. The recombinant protein lacks the C-terminal 114 amino acids but is fully active. PTP1B will dephosphorylate a wide variety of phosphoproteins, such as receptors for the growth factors insulin and epidermal growth factor (EGF), c-Src and beta -catenin. Of particular interest is the observation that PTP1B knock-out mice are resistant to high-caloric intake-induced obesity and have exaggerated insulin responses, suggesting that PTP1B may play an important role in regulating growth factor responsiveness.
- Angers-Loustau, et al. (1999) Biochem. Cell Biol. 77:493.
- Sarmiento, et al. (1998) J. Biol. Chem. 273:26368.
- Bjorge, et al. (2000) J. Biol. Chem. 52:41439.
- Balsamo, et al. (1996) J. Cell Biol. 134:801.
- Elchebly, et al. (1999) Science 283:1544.
Product Datasheets
Citations for Human PTP1B Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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The pseudophosphatase phogrin enables glucose-stimulated insulin signaling in pancreatic ?-cells
Authors: S Torii, C Kubota, N Saito, A Kawano, N Hou, M Kobayashi, R Torii, M Hosaka, T Kitamura, T Takeuchi, H Gomi
J. Biol. Chem., 2018-02-26;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Inhibition of PTP1B disrupts cell-cell adhesion and induces anoikis in breast epithelial cells
Authors: B Hilmarsdot, E Briem, S Halldorsso, J Kricker, S Ingthorsso, S Gustafsdot, GM Mælandsmo, MK Magnusson, T Gudjonsson
Cell Death Dis, 2017-05-11;8(5):e2769.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Selective activation of SHP2 activity by cisplatin revealed by a novel chemical probe-based assay.
Authors: Kuo CC, Chu CY, Lin JJ, Lo LC
Biochem. Biophys. Res. Commun., 2009-11-10;391(1):230-4.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Cysteine S-nitrosylation protects protein-tyrosine phosphatase 1B against oxidation-induced permanent inactivation.
Authors: Chen YY, Chu HM, Pan KT, Teng CH, Wang DL, Wang AH, Khoo KH, Meng TC
J. Biol. Chem., 2008-10-07;283(50):35265-72.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation -
Protein cysteine sulfinic acid reductase (sulfiredoxin) as a regulator of cell proliferation and drug response.
Authors: Lei K, Townsend DM, Tew KD
Oncogene, 2008-05-05;27(36):4877-87.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation, Western Blot -
Calpain 2 and PTP1B function in a novel pathway with Src to regulate invadopodia dynamics and breast cancer cell invasion.
Authors: Cortesio CL, Chan KT, Perrin BJ, Burton NO, Zhang S, Zhang ZY, Huttenlocher A
J. Cell Biol., 2008-03-10;180(5):957-71.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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