Human RAB25 Antibody

Catalog #: AF6605 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6605
AF6605-SP

Detection of Human RAB25 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human RAB25 Antibody Summary

Species Reactivity

Human

Specificity

Detects human RAB25 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human RAB25
Met1-Ala195
Accession # P57735

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Immunogen affinity purified

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human RAB25 by Western Blot. Western blot shows lysates of HT-29 human colon adenocarcinoma cell line, COLO 205 human colorectal adenocarcinoma cell line, and SK-BR-3 human breast cancer cell line. PVDF Membrane was probed with 2 µg/mL of Human RAB25 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6605) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for RAB25 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Rab25

Ras-related protein Rab25, also known as CATX-8, is a 23 kDa (unglycosylated) member of the small GTPase superfamily and Rab family of proteins. Human Rab25 is 213 amino acids (aa) in length. The mature chain runs from aa 1-210 with residues 211-213 constituting a propeptide. Residues 10-174 are noted to be Rab11-like. Rab11a, Rab11b, and Rab25 are closely related Rab proteins that are differentially expressed. Human Rab25 shares 96% aa sequence identity with mouse Rab25. Functionally, Rab25 is involved in the regulation of cell survival. Researchers have shown that Rab25 promotes invasive migration of cells and increases the rates and aggressiveness of breast and ovarian cancers. It is expressed in breast and ovarian tissues.

Long Name

RAs Genes from Brain Protein 25

Entrez Gene IDs

57111 (Human); 53868 (Mouse); 310632 (Rat)

Alternate Names

CATX8; CATX-8RAB11C; RAB11C; Rab25; RAB25, member RAS oncogene family; ras-related protein Rab-25