Human Rab27a Antibody Summary
Ser135-Ala218
Accession # P51159
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Rab27a by Western Blot. Western blot shows lysates of human prostate tissue. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human Rab27a Monoclonal Antibody (Catalog # MAB7245) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Rab27a at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Rab27a in SK‑Mel‑28 Human Cell Line. Rab27a was detected in immersion fixed SK-Mel-28 human malignant melanoma cell line (left panel; positive staining) and Daudi human Burkitt's lymphoma cell line (right panel; negative staining) using Rabbit Anti-Human Rab27a Monoclonal Antibody (Catalog # MAB7245) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Detection of Human Rab27a by Simple WesternTM. Simple Western lane view shows lysates of K562 human chronic myelogenous leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for Rab27a at approximately 31 kDa (as indicated) using 20 µg/mL of Rabbit Anti-Human Rab27a Monoclonal Antibody (Catalog # MAB7245). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Rab27a
RAB27A (Ras-related protein Rab 27A; also GTP-binding protein Ram) is a 27-28 kDa member of the Rab27 subfamily, Rab family, Small GTPase superfamily of proteins. It is widely expressed, and found in cells diverse as mast cells, cytotoxic T cells, melanocytes, retinal pigment epithelium and pancreatic beta -cells. RAB27A plays a key role in the secretion of specialized lysosomes termed secretory lysosomes. In melanocytes, for example, RAB27A is incorporated into the melanosome membrane where it serves as a docking factor for melanophilin and myosin-Va, regulating melanosome transport to, and concentration at, sites of release. Human RAB27A is 221 amino acids (aa) in length. It contains multiple Rab family and subfamily motifs, and concludes with a C-terminal CXC prenylation sequence (aa 219‑221). There is one potential splice variant that shows a deletion of aa 146-153. Over aa 135-218, human RAB27A shares 92% and 94% aa sequence identity with mouse Rab27A and rat RAB27A, respectively.
Product Datasheets
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