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Human/Rat CCR8 PE-conjugated Antibody

Catalog #: FAB1429P
5 Citations Datasheet / COA / SDS
Clone 191704 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
FAB1429P-025
FAB1429P-100
Detection of CCR8 in Human PBMCs by Flow Cytometry.
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Product Details
Citations (5)
FAQs
Supplemental Products
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Rat CCR8 PE-conjugated Antibody Summary

Species Reactivity
Human, Rat
Specificity
Detects human CCR8. Neutralizes the bioactivity of human CCR8 and specifically stains human CCR8-transfected cell lines but not with the parent cell lines.
Source
Monoclonal Rat IgG2B Clone # 191704
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
BaF3 mouse pro-B cell line transfected with human CCR8
Met1-Leu355
Accession # P51685
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)
Purity
Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CCR8 antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of CCR8 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human Integrin aM/CD11b Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB16991G) and either (A) Rat Anti-Human/Rat CCR8 PE-conjugated Monoclonal Antibody (Catalog # FAB1429P) or (B) Rat IgG2BPhycoerythrin Isotype Control (Catalog # IC013P). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of CCR8 antibody in Rat Splenocytes antibody by Flow Cytometry. View Larger

Detection of CCR8 in Rat Splenocytes by Flow Cytometry. Rat splenocytes were stained with Rat Anti-Human/Rat CCR8 PE-conjugated Monoclonal Antibody (Catalog # FAB1429P). Quadrant markers were set based on control antibody staining (Catalog # IC013P). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR8

CCR8 (C-C Chemokine Receptor 8), previously called CKR-L1 or TER1 and designated CD198, is a 41-43 kDa member of the GPCR 1 family of transmembrane proteins.  CCR8 is expressed on vascular smooth muscle cells, monocytes, eosinophils, peritoneal macrophages, thymocytes, CD8+ T cells, Langerhans cells and neurons.  CCL1/TCA3 and vMIP-1 are known agonists for CCR8.  Human CCR8 is a 7-transmembrane protein that is 355 amino acids (aa) in length.  It contains a 35 aa N-terminal extracellular domain plus a 51 aa C-terminal cytoplasmic tail. Over aa sequences 1-33 and 92-105 collectively, human and mouse CCR8 share 64% aa sequence identity.

Entrez Gene IDs
1237 (Human); 12776 (Mouse)
Alternate Names
CC chemokine receptor 8; C-C CKR-8; CC-chemokine receptor chemr1; CC-CKR-8; CCR8; CCR-8; CD198; CDw198 antigen; CDw198; chemokine (C-C motif) receptor 8; chemokine (C-C) receptor 8; chemokine (C-C) receptor-like 2; Chemokine receptor-like 1; ChemR1; CKRL1; CKR-L1; CKRL1MGC129966; CMKBR8MGC129973; CMKBRL2C-C chemokine receptor type 8; CY6; GPRCL1; GPRCY6; GPR-CY6; TER-1; TER1CC chemokine receptor CHEMR1

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Citations for Human/Rat CCR8 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Glatiramer Acetate, Dimethyl Fumarate, and Monomethyl Fumarate Upregulate the Expression of CCR10 on the Surface of Natural Killer Cells and Enhance Their Chemotaxis and Cytotoxicity
    Authors: Zaidoon Al-Jaderi
    Front Immunol, 2016-10-19;7(0):437.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Distinct roles for CCR4 and CXCR3 in the recruitment and positioning of regulatory T cells in the inflamed human liver.
    Authors: Oo YH, Weston CJ, Lalor PF, Curbishley SM, Withers DR, Reynolds GM, Shetty S, Harki J, Shaw JC, Eksteen B, Hubscher SG, Walker LS, Adams DH
    J. Immunol., 2010-02-17;184(6):2886-98.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. CD56highCD16-CD62L- NK cells accumulate in allergic contact dermatitis and contribute to the expression of allergic responses.
    Authors: Carbone T, Nasorri F, Pennino D
    J. Immunol., 2009-12-11;184(2):1102-10.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Chemokine-mediated migration of skin-derived stem cells: predominant role for CCL5/RANTES.
    Authors: Kroeze KL, Jurgens WJ, Doulabi BZ, van Milligen FJ, Scheper RJ, Gibbs S
    J. Invest. Dermatol., 2009-01-01;129(6):1569-81.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Down-modulation of CXCR3 surface expression and function in CD8+ T cells from cutaneous T cell lymphoma patients.
    Authors: Winter D, Moser J, Kriehuber E, Wiesner C, Knobler R, Trautinger F, Bombosi P, Stingl G, Petzelbauer P, Rot A, Maurer D
    J. Immunol., 2007-09-15;179(6):4272-82.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

FAQs

  1. Are there special considerations for Flow Cytometry using Human CCR8 Antibody, Catalog # MAB1429 and FAB1429?

    • The staining with this antibody is temperature dependent;  it will stain at 4 C and room temperature, however, staining is stronger at 37 C.

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