Human/Rat/Hamster ACE-2 Antibody Summary
Gln18-Ser740 (predicted)
Accession # Q9BYF1
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human ACE‑2 by Western Blot. Western blot shows lysates of NS0 mouse myeloma cell line and human kidney tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ACE-2 Monoclonal Antibody (Catalog # MAB9331) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ACE-2 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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ACE‑2 in Rat Kidney. ACE‑2 was detected in immersion fixed frozen sections of rat kidney using Mouse Anti-Human ACE‑2 Monoclonal Antibody (Catalog # MAB9331) at 1 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007). Specific staining was localized to convoluted tubules. Staining was performed using our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
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ACE‑2 in Hamster Lung. ACE‑2 was detected in immersion fixed paraffin-embedded sections of hamster lung using Mouse Anti-Human ACE‑2 Monoclonal Antibody (Catalog # MAB9331) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to respiratory bronchioles. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ACE-2
Angiogensin I Converting Enzyme-2 (ACE-2) is a type I transmembrane zinc protease that cleaves angiotensins I and II to produce vasodilatory and anti-proliferative peptides. The balance between ACE-1 and ACE-2 activity is critical for maintaining cardiovascular, renal, and pulmonary function. ACE-2 isoforms of 75 kDa and 120 kDa are differentially expressed between lung and kidney, respectively, and a shed soluble form is generated by TACE/ADAM17 mediated cleavage. ACE-2 also functions as the cellular uptake receptor for the SARS coronoavirus. Within the extracellular domain, human ACE-2 shares 83% aa sequence identity with mouse and rat ACE-2.
Product Datasheets
Citations for Human/Rat/Hamster ACE-2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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Protein Expression Profile of ACE2 in the Normal and COVID-19-Affected Human Brain
Authors: Cecilia Lindskog, Loren Méar, Johan Virhammar, David Fällmar, Eva Kumlien, Göran Hesselager et al.
Journal of Proteome Research
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ACE2-like carboxypeptidase B38-CAP protects from SARS-CoV-2-induced lung injury
Authors: T Yamaguchi, M Hoshizaki, T Minato, S Nirasawa, MN Asaka, M Niiyama, M Imai, A Uda, JF Chan, S Takahashi, J An, A Saku, R Nukiwa, D Utsumi, M Kiso, A Yasuhara, VK Poon, CC Chan, Y Fujino, S Motoyama, S Nagata, JM Penninger, H Kamada, KY Yuen, W Kamitani, K Maeda, Y Kawaoka, Y Yasutomi, Y Imai, K Kuba
Nature Communications, 2021-11-23;12(1):6791.
Species: Hamster
Sample Types: Tissue Homogenate
Applications: Western Blot -
Highly susceptible SARS-CoV-2 model in CAG promoter-driven hACE2 transgenic mice
Authors: MN Asaka, D Utsumi, H Kamada, S Nagata, Y Nakachi, T Yamaguchi, Y Kawaoka, K Kuba, Y Yasutomi
JCI Insight, 2021-10-08;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Identification of critical determinants on ACE2 for SARS-CoV entry and development of a potent entry inhibitor.
Authors: Han DP, Penn-Nicholson A, Cho MW
Virology, 2006-02-28;350(1):15-25.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Characterization of cytokine/chemokine profiles of severe acute respiratory syndrome.
Authors: Jiang Y, Xu J, Zhou C, Wu Z, Zhong S, Liu J, Luo W, Chen T, Qin Q, Deng P
Am. J. Respir. Crit. Care Med., 2005-01-18;171(8):850-7.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Immune Dysregulation in Acute SARS-CoV-2 Infection
Authors: Lauren Grimm, Chinyere Onyeukwu, Grace Kenny, Danielle M. Parent, Jia Fu, Shaurya Dhingra et al.
Pathogens and Immunity
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