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Human Reg3A Antibody

Catalog #: AF5940 4 Reviews Datasheet / COA / SDS
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AF5940
AF5940-SP
Detection of Human Reg3A by Western Blot.
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Reg3A Antibody Summary

Species Reactivity
Human
Specificity
Detects human Reg3A in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) Reg3G, recombinant mouse (rm) Reg3A, rmReg3B and recombinant rat Reg2 is observed and less than 2% cross-reactivity with rhReg1A, rhReg1B, rmRegIV, rmReg2 and rmReg3D.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Reg3A
Glu27-Asp175
Accession # Q06141
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Purity
Immunogen affinity purified

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Reg3A antibody by Western Blot. View Larger

Detection of Human Reg3A by Western Blot. Western blot shows lysates of human small intestine tissue. PVDF membrane was probed with 1 µg/mL of Human Reg3A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5940) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Reg3A at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Reg3A

Reg3A (regenerating gene III alpha ) is a secreted, 17 kDa, type III member of the Reg family, C-type lectin superfamily of proteins. It is expressed in intestine and exocrine pancreas, where it may have a protective effect against infection and TNF-alpha -induced stress. Mature mouse Reg3A is 149 amino acids (aa) in length (aa 27‑175). It is essentially a soluble C-type lectin domain (aa 40‑173). There are four type III genes ( alpha -δ) in the mouse Reg family. Reg3A has less that 60% aa identity to beta -δ. Mature mouse Reg3A is 87% and 61% aa identical to rat and human Reg3A, respectively.

Long Name
Regenerating Islet-derived IIIa
Entrez Gene IDs
5068 (Human); 19694 (Mouse); 171162 (Rat)
Alternate Names
hepatocarcinoma-intestine-pancreas; HIPpancreatitis-associated protein; Human proislet peptide; Lithostathine 3; pancreatic beta cell growth factor; Pancreatitis-associated protein 1; PAP homologous protein; PAP1FLJ18565; PAP2; PAPPAP-H; PBCGF; proliferation-inducing protein 34; proliferation-inducing protein 42; Reg III-alpha; REG3; Reg3A; REG-3-alpha; regenerating islet-derived 3 alpha; regenerating islet-derived protein 3-alpha; Regenerating islet-derived protein III-alpha; REG-III

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Reviews for Human Reg3A Antibody

Average Rating: 3.3 (Based on 4 Reviews)

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Human Reg3A Antibody
By Anonymous on 01/03/2020
Application: Microarrays Sample Tested: EDTA Plasma Species: Human
Other Human Reg3A Antibody AF5940
Human Reg3A Antibody
By Anonymous on 12/06/2018
Sample Tested: Dried blood spots made with recombinant REG3A from Biovendor Species: Human

Luminex XMAP Platform to develop a newborn screening test for pancreatitis associated protein, to be evaluated with Immuno-ReativeTrypsin in Cystic Fibrosis screening

Human Reg3A Antibody AF5940
Human Reg3A Antibody
By Anonymous on 11/11/2018
Application: ELISA Sample Tested: Serum and Plasma Species: Human
Human Reg3A Antibody
By Anonymous on 11/09/2018
Application: MiAr Sample Tested: EDTA Plasma Species: Human