Human Rex-1/ZFP42 Biotinylated Antibody

Catalog #: BAF3598 Datasheet / COA / SDS

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BAF3598

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Human Rex-1/ZFP42 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Rex‑1/ZFP42 in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Rex‑1/ZFP42
Ser2-Lys150
Accession # NP_777560

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Purity

Antigen Affinity-purified

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Rex‑1

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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Rex‑1 in BG01V Human Stem Cells. Rex‑1 was detected in immersion fixed BG01V human embryonic stem cells using 10 µg/mL Human Rex‑1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF3598) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Rex-1/ZFP42

Rex-1, also named zinc finger protein 42 (Zfp-42), is a 32 kDa transcription factor belonging to the kruppel C2H2-type zinc-finger protein family. It is a putative stem cell marker and is highly expressed in embryonic and adult stem cells. Over the region used for immunization, human and mouse Rex-1 share 47% amino acid sequence identity.

Long Name

Zinc Finger Protein 42

Entrez Gene IDs

132625 (Human); 22702 (Mouse); 290765 (Rat)

Alternate Names

FLJ20086; KIAA1584; MGC21637; MGC61687; Rex1; Rex-1; SUHW4; Suppressor of hairy wing homolog 4; ZFP42; zinc finger protein 280D; Zinc finger protein 634; ZNF634suppressor of hairy wing homolog 4 (Drosophila)