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Human ROBO2 Antibody

Catalog #: AF3147
6 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3147
AF3147-SP
Product Details
Citations (6)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ROBO2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human ROBO2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 2% cross-reactivity with recombinant rat ROBO1, recombinant human (rh) ROBO3 and rhROBO4 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human ROBO2
Ser22-Ala312, RPVA, Pro313-Pro859
Accession # Q9HCK4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Purity
Immunogen affinity purified

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human ROBO2 Fc Chimera (Catalog # 3147-RB)
Flow Cytometry
2.5 µg/106 cells
NTera‑2 human testicular embryonic carcinoma cell line differentiated with retinoic acid
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-15 µg/mL
Immersion fixed human neural progenitor cells differentiated by growth factor withdrawal

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ROBO2

Human ROBO2 is a 175 kDa member of the ROBO/roundabout receptor family and is a 1357 amino acid (aa) type I transmembrane (TM) protein. It contains an extracellular domain (ECD) comprised of five C2-type Ig-like domains and three fibronectin type III domains. ROBO receptors interact with SLIT proteins to regulate axon guidance. Similar to ROBO1, the first two Ig-like domains of ROBO2 are predicted to be important for SLIT binding. Alternate splice forms of ROBO2 exist. The ROBO2 ECD in human shares 98% aa identity with the ROBO2 ECD in mouse and canine.

Long Name
Roundabout Homolog 2
Entrez Gene IDs
6092 (Human); 268902 (Mouse); 84409 (Rat)
Alternate Names
Drosophila) homolog 2; ROBO2; roundabout, axon guidance receptor, homolog 2 (Drosophila); SAX3

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Citations for Human ROBO2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Ndfip Proteins Target Robo Receptors for Degradation and Allow Commissural Axons to Cross the Midline in the Developing Spinal Cord
    Authors: M Gorla, C Santiago, K Chaudhari, AAK Layman, PM Oliver, GJ Bashaw
    Cell Rep, 2019-03-19;26(12):3298-3312.e4.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Immunoprecipitation
  2. The chemorepellent Slit3 promotes monocyte migration.
    Authors: Geutskens SB, Hordijk PL, van Hennik PB
    J. Immunol., 2010-11-15;185(12):7691-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Sequential arrival and graded secretion of Sema3F by olfactory neuron axons specify map topography at the bulb.
    Authors: Takeuchi H, Inokuchi K, Aoki M
    Cell, 2010-06-11;141(6):1056-67.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Manipulating Robo expression in vivo perturbs commissural axon pathfinding in the chick spinal cord.
    Authors: Reeber SL, Sakai N, Nakada Y, Dumas J, Dobrenis K, Johnson JE, Kaprielian Z
    J. Neurosci., 2008-08-27;28(35):8698-708.
    Species: Chicken
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  5. Robos and slits control the pathfinding and targeting of mouse olfactory sensory axons.
    Authors: Nguyen-Ba-Charvet KT, Di Meglio T, Fouquet C, Chedotal A
    J. Neurosci., 2008-04-16;28(16):4244-9.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. The role of Slit-Robo signaling in the generation, migration and morphological differentiation of cortical interneurons.
    Authors: Andrews W, Barber M, Hernadez-Miranda LR, Xian J, Rakic S, Sundaresan V, Rabbitts TH, Pannell R, Rabbitts P, Thompson H, Erskine L, Murakami F, Parnavelas JG
    Dev. Biol., 2007-11-13;313(2):648-58.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC

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