Human RUNX3/CBFA3 Antibody

Catalog #: AF3765 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3765
AF3765-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human RUNX3/CBFA3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human RUNX3/CBFA3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody shows less than 1% cross‑reactivity with recombinant human (rh) CBFA1 and rhCBFA2.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human RUNX3/CBFA3
Lys186-Tyr415
Accession # Q13761

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human RUNX3/CBFA3

Immunocytochemistry

5-15 µg/mL

Immersion fixed Raji human Burkitt's lymphoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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RUNX3/CBFA3 in Raji Human Cell Line. RUNX3/CBFA3 was detected in immersion fixed Raji human Burkitt's lymphoma cell line using Goat Anti-Human RUNX3/CBFA3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3765) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RUNX3/CBFA3

RUNX3, also known as CBFA3 and PEBP2 alpha (polyomavirus enhancer binding protein 2 alpha ) is a member of the runt-related (RUNX) family of transcription factors. It is the DNA binding subunit of the heterodimeric transcription factor CBF/PEBP2. RUNX3 is a nuclear effector of the BMP/TGF-beta pathways and its inactivation or reduced expression is associated with numerous human malignancies. Two isoforms that differ in their N-terminal 5/19 residues have been reported. The 415 amino acid residue of human RUNX3 shares 91% amino acid sequence homology with mouse or rat RUNX3.

Long Name

Runt-related Transcription Factor 3

Entrez Gene IDs

864 (Human); 12399 (Mouse); 156726 (Rat)

Alternate Names

Acute myeloid leukemia 2 protein; AML2; AML2SL3/AKV core-binding factor alpha C subunit; CBFA3; CBFA3MGC16070; CBF-alpha-3; PEA2 alpha C; PEA2-alpha C; PEBP2 alpha C; PEBP2A3; PEBP2A3FLJ34510; PEBP2AC; PEBP2-alpha C; runt domain, alpha subunit 3; runt-related transcription factor 3; RUNX3; SL3-3 enhancer factor 1 alpha C subunit; transcription factor AML2