Human Ryk Biotinylated Antibody

Catalog #: BAF4907 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
BAF4907

All Ryk Antibodies

Product Details

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Ryk Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Ryk in Western blots. In Western blots, approximately 40% cross-reactivity with recombinant mouse Ryk is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Ryk
Ala47-Thr224
Accession # NP_002949

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Ryk

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ryk

RYK (related to tyrosine (Y) kinase; also H-RYK) is a 70-90 kDa, atypical member of the tyrosine protein kinase family. It is a type I transmembrane glycoprotein that binds Wnt, and forms a Wnt receptor complex with frizzled. In this capacity, it serves as a link between Wnt and Dishevelled. Human RYK is 604 amino acids (aa) in length (SwissProt Accession # P34925). It contains a 25 aa signal sequence and a 199 aa extracellular region (aa 26-224) that shows one WIF-1-like domain (aa 63‑191). The cytoplasmic region possesses a nonfunctional Ser/Thr protein kinase domain (aa 327-600). One isoform exists that shows a 31 aa substitution between aa’s 18-46. Over aa 47-224, human RYK is 94% aa identical to mouse RYK.

Long Name

Receptor Type Tyrosine Protein Kinase RYK

Entrez Gene IDs

6259 (Human); 20187 (Mouse); 140585 (Rat)

Alternate Names

D3S3195; EC 2.7.10; EC 2.7.10.1; hydroxyaryl-protein kinase; JTK5; JTK5A protein tyrosine kinase; JTK5A; RYK receptor-like tyrosine kinase; Ryk; RYK1; tyrosine-protein kinase RYK