Human S100A9 Antibody

Catalog #: MAB5578
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5578
MAB5578-SP

Detection of Human S100A9 by Western Blot.

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All S100A9 Antibodies

Product Details

Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human S100A9 Antibody Summary

Species Reactivity

Human

Specificity

Detects human S100A9 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) S100A1, 2, 7, 8, 11, 13, rhS100B, rhS100P, recombinant mouse S100A4, 6, 10, or 16 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 474315

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human S100A9
Met1-Pro114
Accession # P06702

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human S100A9 by Western Blot. Western blot shows lysates of human peripheral blood mononuclear cells (PBMC), human spleen tissue, human tonsil tissue, and human cartilage tissue. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human S100A9 Monoclonal Antibody (Catalog # MAB5578) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for S100A9 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

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S100A9 in Capan-1 Human Cell Line. S100A9 was detected in immersion fixed Capan-1 human pancreatic adenocarcinoma cell line using 10 µg/mL Mouse Anti-Human S100A9 Monoclonal Antibody (Catalog # MAB5578) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: S100A9

Human S100A9 (also MRP-14, Calgranulin-B, and p14) is a 14 kDa member of the S100 family of EF-hand calcium-binding proteins. It is 114 amino acids (aa) in length and contains short sequential modules. There is an N-terminal helical region, followed by a calcium-binding EF-hand domain, two more helical regions, a second EF-hand domain, and three additional helical regions. S100A9 will noncovalently heterodimerize with S100A8. In the presence of calcium, this heterodimer will form a heterotetramer. S100A9 is expressed in granulocytes, monocytes, and macrophages during acute and chronic inflammation. Human S100A9 shares 62% and 57% aa identity with rat and mouse S100A9, respectively.

Long Name

S100 Calcium Binding Protein A9

Entrez Gene IDs

6280 (Human); 20202 (Mouse)

Alternate Names

CAGBMigration inhibitory factor-related protein 14; Calgranulin B; calgranulin-B; Calprotectin L1H subunit; CFAGMRP-14,60B8AG; CGLB; L1AG; LIAG; MAC387; MIF; MRP-14; MRP14Leukocyte L1 complex heavy chain; NIF; P14; protein S100-A9; S100 calcium binding protein A9 (calgranulin B); S100 calcium binding protein A9; S100 calcium-binding protein A9 (calgranulin B); S100 calcium-binding protein A9; S100A9

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human S100A9 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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High-Plex Spatial RNA Profiling Reveals Cell Type‒Specific Biomarker Expression during Melanoma Development
Authors: Maija Kiuru, Michelle A. Kriner, Samantha Wong, Guannan Zhu, Jessica R. Terrell, Qian Li et al.
Journal of Investigative Dermatology
Lipid and protein tumor markers for head and neck squamous cell carcinoma identified by imaging mass spectrometry
Authors: J Schmidt, B Kajtár, K Juhász, M Péter, T Járai, A Burián, L Kereskai, I Gerlinger, T Tornóczki, G Balogh, L Vígh, L Márk, Z Balogi
Oncotarget, 2020-07-14;11(28):2702-2717.
Species: Human
Sample Types: Whole Tissue
Applications: IHC