Human SLITRK6 Antibody

Catalog #: AF7317 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF7317
AF7317-SP

Detection of Human SLITRK6 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human SLITRK6 Antibody Summary

Species Reactivity

Human

Specificity

Detects human SLITRK6 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) SLITRK2, rhSLITRK4, and rhSLITRK6 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human SLITRK6
Ile16-Ala604
Accession # Q9H5Y7

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human SLITRK6 by Western Blot. Western blot shows lysates of SH-SY5Y human neuroblastoma cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human SLITRK6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7317) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SLITRK6 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SLITRK6

SLITRK6 (Slit and NTRK-like 6) is a 95 kDa (predicted) member of the SLITRK family of proteins. It is widely expressed in the embryo, including cells in limb bud mesenchyme, visceral organ primodia, cells of the dorsal thalamus, and cochlear plus vestibular sensory epithelium. In the ear, SLITRK6 is suggested to induce neurotrophin expression from cells, thus promoting sensory neuron survival and axon pathfinding. Mature human SLITRK6 is an 826 amino acid (aa) type I transmembrane protein. It contains a 593 aa extracellular domain (ECD) (aa 16-608) plus a 212 aa cytoplasmic region. The ECD possesses two LRR clusters, one that involves seven LRRs (aa 27-269), and a second that involves eight LRRs (aa 320-568). Over aa 16-604, human SLITRK6 shares 89% aa sequence identity with mouse SLITRK6.

Long Name

SLIT and NTRK-like Family, Member 6

Entrez Gene IDs

84189 (Human); 239250 (Mouse); 290467 (Rat)

Alternate Names

FLJ22774; MGC119595; MGC119596; MGC119597,4832410J21Rik; SLIT and NTRK-like family, member 6; SLIT and NTRK-like protein 6; slit and trk like gene 6; SLITRK6