Human SLPI Antibody

Catalog #: AF1274
11 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF1274
AF1274-SP

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Citations (11)

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Human SLPI Antibody Summary

Species Reactivity

Human

Specificity

Detects human SLPI in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with mouse SLPI is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human SLPI

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human SLPI (Catalog # 1274-PI)

Immunohistochemistry

5-15 µg/mL

See below

Neutralization

Measured by its ability to neutralize Recombinant Human SLPI (0.125 µg/mL, Catalog # 1274-PI) inhibition of Trypsin (0.05 µg/mL) cleavage of the fluorogenic peptide substrate Mca-RPKPVE-Nval-WRK(Dnp)-NH₂ (10 µM, Catalog # ES002). The Neutralization Dose (ND₅₀) is typically 2 µg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

View Larger

SLPI in Human Lung. SLPI was detected in immersion fixed paraffin-embedded sections of human lung using Goat Anti-Human SLPI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1274) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SLPI

SLPI is a member of the Serpin family and is primarily expressed in secretory glandular epithelial cells. It is found in various secretory fluids and functions as a potent inhibitor of many proteolytic enzymes. SLPI can modulate cell proliferation and has been shown to have anti-inflammatory, anti-viral, and anti-bacterial activities (1, 2).

References

Zhang, D. et al. (2002) J. Biol. Chem. 277:29999.
Taggart, C.C. et al. (2002) J. Biol. Chem. 277:33648.

Long Name

Secretory Leukocyte Protease Inhibitor

Entrez Gene IDs

6590 (Human); 20568 (Mouse)

Alternate Names

ALK1; ALPProtease inhibitor WAP4; antileukoproteinase; BLPISeminal proteinase inhibitor; HUSI; HUSI-I; Mucus proteinase inhibitor; secretory leukocyte peptidase inhibitor; secretory leukocyte protease inhibitor (antileukoproteinase); Secretory leukocyte protease inhibitor; SLPI; WAP four-disulfide core domain protein 4; WAP4HUSI-1; WFDC4MPI

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human SLPI Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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Protease-anti-protease compartmentalization in SARS-CoV-2 ARDS: Therapeutic implications
Authors: OF McElvaney, T Asakura, SL Meinig, JL Torres-Cas, RS Hagan, C Gabillard, MP Murphy, LB Thorne, A Borczuk, EP Reeves, RE Zumwalt, Y Mikami, TP Carroll, K Okuda, G Hogan, OJ McElvaney, J Clarke, NL McEvoy, PW Mallon, C McCarthy, G Curley, MC Wolfgang, RC Boucher, NG McElvaney
EBioMedicine, 2022-02-22;77(0):103894.
Species: Human
Sample Types: Plasma
Applications: Western Blot
Characterisation of the Major Extracellular Proteases of Stenotrophomoas maltophilia and Their Effects on Pulmonary Antiproteases
Authors: K Molloy, SG Smith, G Cagney, ET Dillon, CM Greene, NG McElvaney
Pathogens, 2019-06-28;8(3):.
Species: Human
Sample Types: Recombinant Protein
Applications: Western Blot
Profiling human breast epithelial cells using single cell RNA sequencing identifies cell diversity
Authors: QH Nguyen, N Pervolarak, K Blake, D Ma, RT Davis, N James, AT Phung, E Willey, R Kumar, E Jabart, I Driver, J Rock, A Goga, SA Khan, DA Lawson, Z Werb, K Kessenbroc
Nat Commun, 2018-05-23;9(1):2028.
Species: Human
Sample Types: Whole Tissue
Applications: IHC
Secretory leukocyte protease inhibitor reverses inhibition by CNS myelin, promotes regeneration in the optic nerve, and suppresses expression of the transforming growth factor-beta signaling protein Smad2.
Authors: Hannila S, Siddiq M, Carmel J, Hou J, Chaudhry N, Bradley P, Hilaire M, Richman E, Hart R, Filbin M
J Neurosci, 2013-03-20;33(12):5138-51.
Species: Rat
Sample Types: Cell Lysates, Whole Cells
Applications: ChIP, Western Blot
Secretory leukocyte protease inhibitor (SLPI) expression and tumor invasion in oral squamous cell carcinoma.
Authors: Wen J, Nikitakis NG, Chaisuparat R, Greenwell-Wild T, Gliozzi M, Jin W, Adli A, Moutsopoulos N, Wu T, Warburton G, Wahl SM
Am. J. Pathol., 2011-06-01;178(6):2866-78.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P
The major bactericidal activity of human seminal plasma is zinc-dependent and derived from fragmentation of the semenogelins.
Authors: Edstrom AM, Malm J, Frohm B, Martellini JA, Giwercman A, Morgelin M, Cole AM, Sorensen OE
J. Immunol., 2008-09-01;181(5):3413-21.
Species: Human
Sample Types: Seminal Plasma
Applications: Western Blot
Use of an immunoaffinity-mass spectrometry-based approach for the quantification of protein biomarkers from serum samples of lung cancer patients.
Authors: Nicol GR, Han M, Kim J, Birse CE, Brand E, Nguyen A, Mesri M, FitzHugh W, Kaminker P, Moore PA, Ruben SM, He T
Mol. Cell Proteomics, 2008-04-03;7(10):1974-82.
Species: Human
Sample Types: Serum
Applications: Mass Spectrometry
The secretory leukocyte protease inhibitor (SLPI) and the secondary granule protein lactoferrin are synthesized in myelocytes, colocalize in subcellular fractions of neutrophils, and are coreleased by activated neutrophils.
Authors: Jacobsen LC, Sorensen OE, Cowland JB, Borregaard N, Theilgaard-Monch K
J. Leukoc. Biol., 2008-02-19;83(5):1155-64.
Species: Human
Sample Types: Cell Culture Supernates, Whole Cells
Applications: ICC, Western Blot
The alarm anti-protease, secretory leukocyte protease inhibitor, is a proliferation and survival factor for ovarian cancer cells.
Authors: Simpkins FA, Devoogdt NM, Rasool N, Tchabo NE, Alejandro EU, Kamrava MM, Kohn EC
Carcinogenesis, 2007-10-04;29(3):466-72.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
cDNA microarray analysis of invasive and tumorigenic phenotypes in a breast cancer model.
Authors: Kluger HM, Kluger Y, Gilmore-Hebert M, DiVito K, Chang JT, Rodov S, Mironenko O, Kacinski BM, Perkins AS, Sapi E
Lab. Invest., 2004-03-01;84(3):320-31.
Species: Human
Sample Types: Whole Cells
Applications: ICC

Antimicrobial components of vaginal fluid.
Authors: Valore EV, Park CH, Igreti SL, Ganz T
Am. J. Obstet. Gynecol., 2002-09-01;187(3):561-8.
Species: Human
Sample Types: Vaginal Fluid
Applications: Western Blot