Human SMAC/Diablo Antibody Summary
Ala56-Asp239
Accession # Q9NR28
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human SMAC/Diablo by Western Blot. Western blot shows lysates of SH-SY5Y human neuroblastoma cell line, CHP-100 human neuroblastoma cell line, and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 0.5 µg/mL Rabbit Anti-Human SMAC/Diablo Antigen Affinity-purified Polyclonal Antibody (Catalog # AF789) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). For additional reference, Recombinant Human SMAC/Diablo (Catalog # 789-SM) (0.5 ng/lane) was included. A specific band for SMAC/Diablo was detected at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 6.
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Detection of Human SMAC/Diablo by Simple WesternTM. Simple Western lane view shows lysates of SH‑SY5Y human neuroblastoma cell line and Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for SMAC/Diablo at approximately 29 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human SMAC/Diablo Antigen Affinity-purified Polyclonal Antibody (Catalog # AF789). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SMAC/Diablo
SMAC (second mitochondria derived activator of caspase)/Diablo promotes caspase activation by interacting with the inhibitor of apoptosis (IAP) proteins in the cytochrome c/Apaf-1/caspase-9 pathway.
- Du, C. et al. (2000) Cell 102:33.
- Chai, J. et al. (2000) Nature 406:855.
- Srinivasula, S. et al. (2000) J. Biol. Chem. 275:36152.
- Ekert, P. et al. (2001) J. Cell Biol. 152:483.
- Verhagen, A. (2000) Cell 102:43.
Product Datasheets
Citations for Human SMAC/Diablo Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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Pro-Apoptotic Activity of New Honokiol/Triphenylmethane Analogues in B-Cell Lymphoid Malignancies
Molecules, 2016-07-30;21(8):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Proteasome inhibition can impair caspase-8 activation upon submaximal stimulation of apoptotic tumor necrosis factor-related apoptosis inducing ligand (TRAIL) signaling.
Authors: Laussmann MA, Passante E, Hellwig CT
J. Biol. Chem., 2012-03-09;287(18):14402-11.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Role and regulation of nodal/activin receptor-like kinase 7 signaling pathway in the control of ovarian follicular atresia.
Authors: Wang H, Jiang JY, Zhu C, Peng C, Tsang BK
Mol. Endocrinol., 2006-05-18;20(10):2469-82.
Species: Rat
Sample Types: Cell Lysates
Applications: Western Blot -
Inhibition of lymphotoxin-beta receptor-mediated cell death by survivin-DeltaEx3.
Authors: You RI, Chou YC
Cancer Res., 2006-03-15;66(6):3051-61.
Species: Human
Sample Types: Whole Cells
Applications: ICC
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