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Human SMAC/Diablo Antibody

Catalog #: AF7891
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF7891
AF7891-SP
SMAC/Diablo in Human Prostate.
2 Images
Product Details
Citations (2)
FAQs
Supplemental Products
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human SMAC/Diablo Antibody Summary

Species Reactivity
Human
Specificity
Detects human, mouse, and rat SMAC/Diablo in direct ELISAs and Western blots. No cross-reactivity with recombinant human NME3 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human SMAC/Diablo
Ala56-Asp239
Accession # Q9NR28
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry SMAC/Diablo antibody in Human Prostate by Immunohistochemistry (IHC-P). View Larger

SMAC/Diablo in Human Prostate. SMAC/Diablo was detected in immersion fixed paraffin-embedded sections of human prostate using Goat Anti-Human SMAC/Diablo Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7891) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of glandular epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Western Blot Detection of Human SMAC/Diablo antibody by Western Blot. View Larger

Detection of Human SMAC/Diablo by Western Blot. Western blot shows lysates of SH-SY5Y human neuroblastoma cell line, mouse NB41A3 mouse neuroblastoma cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human SMAC/Diablo Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7891) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for SMAC/Diablo at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SMAC/Diablo

SMAC (second mitochondria derived activator of caspase)/Diablo promotes caspase activation by interacting with the inhibitor of apoptosis (IAP) proteins in the cytochrome c/Apaf-1/caspase-9 pathway.

References
  1. Du, C. et al. (2000) Cell 102:33.
  2. Chai, J. et al. (2000) Nature 406:855.
  3. Srinivasula, S. et al. (2000) J. Biol. Chem. 275:36152.
  4. Ekert, P. et al. (2001) J. Cell Biol. 152:483.
  5. Verhagen, A. (2000) Cell 102:43.
Long Name
Second Mitochondria-derived Activator of Caspase
Entrez Gene IDs
56616 (Human)
Alternate Names
0610041G12Rik; Diablo; diablo, IAP-binding mitochondrial protein; Direct IAP-binding protein with low pI; mitochondrial Smac protein; Second mitochondria-derived activator of caspase; SMAC; SMACmitochondrial

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Citations for Human SMAC/Diablo Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Cytotoxic activity of the amphibian ribonucleases onconase and r-amphinase on tumor cells from B cell lymphoproliferative disorders.
    Authors: Smolewski P, Witkowska M, Zwolinska M, Cebula-Obrzut B, Majchrzak A, Jeske A, Darzynkiewicz Z, Ardelt W, Ardelt B, Robak T
    Int J Oncol, 2014-04-28;45(1):419-25.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Motoneuron resistance to apoptotic cell death in vivo correlates with the ratio between X-linked inhibitor of apoptosis proteins (XIAPs) and its inhibitor, XIAP-associated factor 1.
    Authors: Perrelet D, Perrin FE, Liston P, Korneluk RG, MacKenzie A, Ferrer-Alcon M, Kato AC
    J. Neurosci., 2004-04-14;24(15):3777-85.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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