Human SNAP23 Antibody

Catalog #: AF6306 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6306
AF6306-SP

Detection of Human SNAP23 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human SNAP23 Antibody Summary

Species Reactivity

Human

Specificity

Detects human SNAP23 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse SNAP23 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human SNAP23
Asp146-Ser211
Accession # O00161

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human SNAP23 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human SNAP23 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6306) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SNAP23 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

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SNAP23 in HepG2 Human Cell Line. SNAP23 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Sheep Anti-Human SNAP23 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6306) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SNAP23

SNAP23 (Synaptosomal associated protein 23; also Syndet) is a membrane-associated 23‑29 kDa member of the SNAP25 family of proteins. It is expressed in multiple cell types, including neutrophils, platelets, mast cells and adipocytes. SNAP23 is involved in vesicle exocytosis. It has been proposed that SNAP23 can associate with both vesicle and target (cell) membranes via a lipid modification. As a v-SNARE, it may interact with syntaxin-6 at the cell membrane. As a t-SNARE, in conjunction with syntaxin-4, it likely interacts with VAMP-2 and -8 on the vesicle membrane. In either case, this approximates two membranes, which subsequently fuse to create a pore. Human SNAP23 is 211 amino acids (aa) in length. It contains two t-SNARE coiled-coil homology domains (aa 14‑76 and 148‑207). Palmitoylation occurs between aa 80‑87, while phosphorylation occurs on multiple Ser/Thr residues. There is one splice variant that shows a deletion of aa 90‑142. Over aa 146‑211, human and mouse SNAP23 share 85% aa identity.

Long Name

Synaptosomal-associated Protein, 23kDa

Entrez Gene IDs

8773 (Human); 20619 (Mouse); 64630 (Rat)

Alternate Names

HsT17016; SNAP23; SNAP-23; SNAP23A; SNAP23B; synaptosomal-associated protein 23; synaptosomal-associated protein, 23kD; synaptosomal-associated protein, 23kDa; Vesicle-membrane fusion protein SNAP-23