Human SOCS-5 Antibody

Catalog #: AF4796 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4796
AF4796-SP

Detection of Human SOCS‑5 by Western Blot.

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All SOCS-5 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human SOCS-5 Antibody Summary

Species Reactivity

Human

Specificity

Detects human SOCS-5 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human SOCS‑1, -2, -3, -6, and -7 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human SOCS-5
Met1-Lys536
Accession # O75159

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human SOCS‑5 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human SOCS-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4796) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for SOCS-5 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunocytochemistry

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SOCS‑5 in Jurkat Human Cell Line. SOCS-5 was detected in immersion fixed Jurkat human acute T cell leukemia cell line using Goat Anti-Human SOCS-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4796) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SOCS-5

SOCS-5 (Suppressor of cytokine signaling 5; also CIS-6) is a 55‑60 kDa member of the SOCS family of proteins. It is an SH2-domain containing cytoplasmic adaptor protein expressed by Th1 cells that is known to reduce the expression of the EGF receptor and block signaling by the IL-4 R alpha chain. Human SOCS-5 is 536 amino acids (aa) in length. It contains one SH2 domain (aa 381‑476) and a SOCS box (aa 471‑520) that mediates interaction with E3 ubiquitin ligase complexes. Full-length SOCS-5 is 94% aa identical to mouse SOCS-5.

Long Name

Suppressor Of Cytokine Signaling 5

Entrez Gene IDs

9655 (Human); 56468 (Mouse); 500616 (Rat)

Alternate Names

CIS6; CIS6Cytokine-inducible SH2 protein 6; CISH5; CISH6; CISH6CIS-6; KIAA0671Cytokine-inducible SH2-containing protein 5; SOCS5; SOCS-5; SOCS-5CISH5; suppressor of cytokine signaling 5