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Human SOD3/EC-SOD Antibody

Catalog #: MAB34201
1 Citation Datasheet / COA / SDS
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MAB34201
MAB34201-SP
Detection of Human SOD3/EC‑SOD by Western Blot.
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human SOD3/EC-SOD Antibody Summary

Species Reactivity
Human
Specificity
Detects human SOD3/EC-SOD in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human SOD2, recombinant mouse (rm) SOD1, or rmSOD3 is observed.
Source
Monoclonal Mouse IgG1 Clone # 713707
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human SOD3/EC-SOD
Trp19-Ala240
Accession # P08294
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human SOD3/EC-SOD antibody by Western Blot. View Larger

Detection of Human SOD3/EC‑SOD by Western Blot. Western blot shows lysates of human aorta tissue. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human SOD3/EC-SOD Monoclonal Antibody (Catalog # MAB34201) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for SOD3/EC-SOD at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SOD3/EC-SOD

Superoxide Dismutases (SODs), originally identified as Indophenoloxidase (IPO), are enzymes that catalyze the conversion of naturally-occuring, but harmful, superoxide radicals into molecular oxygen and hydrogen peroxide. Superoxide Dismutase 3 (SOD3) also known as extracellular (EC) SOD, is tetrameric glycoprotein with an apparent subunit molecular weight of about 30 kDa. Three isoenzymes of SOD have been identified and are functionally related but have very modest sequence homology. SOD3 shares 23% and 17% sequence identity with SOD1 and SOD2, respectively. Human SOD3 shares ~64% sequence homology with mouse and rat SOD3. Like SOD1, SOD3 binds one Cu2+ and Zn2+ ions per subunit but differs in sequence and tissue distribution. SOD3 is a secretory protein and is synthesized with a putative 18-amino acid signal peptide preceding the 222 amino acids in the mature SOD3. SOD3 is found in plasma, lymph, and synovial fluid as well as in tissues. SOD3 binds on the surface of endothelial cells through the heparan sulfate proteoglycan and eliminates the oxygen radicals from the NADP‑dependent oxidative system of neutrophils.

Long Name
Superoxide Dismutase-3
Entrez Gene IDs
6649 (Human); 20657 (Mouse); 25352 (Rat)
Alternate Names
ECSOD; EC-SOD; EC-SODEC 1.15.1.1; extracellular superoxide dismutase [Cu-Zn]; MGC20077; SOD3; superoxide dismutase 3, extracellular

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Citation for Human SOD3/EC-SOD Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Human Neonatal Thymus Mesenchymal Stem/Stromal Cells and Chronic Right Ventricle Pressure Overload
    Authors: Josue Chery, Shan Huang, Lianghui Gong, Shuyun Wang, Zhize Yuan, Joshua Wong et al.
    Bioengineering (Basel)

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