Human SOD3/EC-SOD Antibody

Catalog #: AF3420
3 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3420
AF3420-SP

Detection of Human SOD3/EC‑SOD by Western Blot.

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All SOD3/EC-SOD Antibodies

Product Details

Citations (3)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human SOD3/EC-SOD Antibody Summary

Species Reactivity

Human

Specificity

Detects human SOD3/EC‑SOD in Western blots. In Western blots, no cross-reactivity with recombinant human (rh) SOD1 or rhSOD2 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human SOD3/EC‑SOD
Trp19-Ala240
Accession # P08294

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.2 µg/mL

See below

Simple Western

10 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human SOD3/EC‑SOD by Western Blot. Western blot shows lysates of human aorta tissue. PVDF Membrane was probed with 0.2 µg/mL of Goat Anti-Human SOD3/EC-SOD Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3420) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for SOD3/EC-SOD at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Immunohistochemistry

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SOD3/EC‑SOD in Human Kidney. SOD3/EC-SOD was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human SOD3/EC-SOD Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3420) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of epithelial cells in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western

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Detection of Human SOD3/EC‑SOD by Simple Western^TM. Simple Western lane view shows lysates of human kidney tissue, loaded at 0.2 mg/mL. A specific band was detected for SOD3/EC-SOD at approximately 38 kDa (as indicated) using 10 µg/mL of Goat Anti-Human SOD3/EC-SOD Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3420) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SOD3/EC-SOD

Superoxide Dismutases (SODs), originally identified as Indophenoloxidase (IPO), are enzymes that catalyze the conversion of naturally-occuring, but harmful, superoxide radicals into molecular oxygen and hydrogen peroxide. Superoxide Dismutases 3, SOD3, also known as extracellular (EC) SOD, is tetrameric glycoprotein with an apparent subunit molecular weight of about 30 kDa. Three isoenzymes of SOD have been identified and are functionally related but have very modest sequence homology. SOD3 shares 23% and 17% sequence identity with SOD1 and SOD2, respectively. SOD3 shares ~64% sequence homology with mouse and rat SOD3. Like SOD1, SOD3 binds one Cu²⁺ and Zn²⁺ ions per subunit but differs in sequence and tissue distribution. SOD3 is a secretory protein and is synthesized with a putative 18-amino acid signal peptide preceding the 222 amino acids in the mature SOD3. SOD3 is found in plasma, lymph, and synovial fluid as well as in tissues. SOD3 binds on the surface of endothelial cells through the heparan sulfate proteoglycan and eliminates the oxygen radicals from the NADP-dependent oxidative system of neutrophils.

Long Name

Superoxide Dismutase-3

Entrez Gene IDs

6649 (Human); 20657 (Mouse); 25352 (Rat)

Alternate Names

ECSOD; EC-SOD; EC-SODEC 1.15.1.1; extracellular superoxide dismutase [Cu-Zn]; MGC20077; SOD3; superoxide dismutase 3, extracellular

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human SOD3/EC-SOD Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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GATA3 induces mitochondrial biogenesis in primary human CD4+ T cells during DNA damage
Authors: LA Callender, J Schroth, EC Carroll, C Garrod-Ket, LEL Romano, E Hendy, A Kelly, P Lavender, AN Akbar, JP Chapple, SM Henson
Nature Communications, 2021-06-07;12(1):3379.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
SOD3 improves the tumor response to chemotherapy by stabilizing endothelial HIF-2?
Authors: E Mira, L Carmona-Ro, B Pérez-Vill, J Casas, MJ Fernández-, D Martínez-R, P Martín-Gon, I Heras-Muri, M Paz-Cabeza, M Tardáguila, TD Oury, S Martín-Pui, RA Lacalle, G Fabriás, E Díaz-Rubio, S Mañes
Nat Commun, 2018-02-08;9(1):575.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P
Rejuvenation of mesenchymal stem cells by extracellular vesicles inhibits the elevation of reactive oxygen species
Authors: Vuong Cat Khanh, Toshiharu Yamashita, Kinuko Ohneda, Chiho Tokunaga, Hideyuki Kato, Motoo Osaka et al.
Scientific Reports