Human ST7/LRP12 Antibody

Catalog #: AF2560 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2560
AF2560-SP

All ST7/LRP12 Antibodies

Product Details

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ST7/LRP12 Antibody Summary

Species Reactivity

Human

Specificity

Detects human ST7/LRP12 in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant mouse ST7/LRP12 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human ST7/LRP12
Asn28-Ile488
Accession # Q9Y561

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human ST7/LRP12 (Catalog # 2560-S7)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ST7/LRP12

ST7 (Suppressor of Tumorigenicity 7), also known as RAY1, TSG7 and FAM4A1, is a type I transmembrane protein belonging to the LDLR superfamily and is designated LRP12 (1-3). The human ST7 cDNA encodes 859 amino acids (aa) including a 32 aa signal sequence, a 460 aa extracellular domain (ECD) containing two CUB domains and five LDLR class A domains, a 21 aa transmembrane domain, and a 346 aa cytoplasmic domain containing motifs implicated in endocytosis and signal transduction (1, 2). Human ST7 shares 95% aa sequence homology with mouse and rat, 96% with canine, and 98% with bovine, equine and porcine ST7 within the ECD. Genomic sequencing indicates the possibility of up to 18 splicing isoforms, but expression of these has not been well-studied (3). ST7 is widely expressed in normal tissues, especially fibroblasts (1, 4). Highest mRNA levels were detected in heart and skeletal muscle (1). ST7 was originally proposed to be a tumor suppressor protein, but it is not consistently down‑regulated in a variety of cancers, either by mutation or loss of heterozygosity (1, 4-7). In certain cancers, expression may even be up‑regulated (8). Expression may be associated with down‑regulated expression of extracellular matrix molecules that are involved in remodeling, such as SPARC, IGFBP5 and several matrix metalloproteinases, and modulation of in vivo tumorigenicity (4, 5).

References

Qing, J. et al. (1999) Oncogene 18:335.
Battle, M.A. et al. (2003) Biochemistry 42:7270.
Vincent, J.B. et al. (2002) Genomics 80:283.
Zenclusen, J.C. et al. (2001) Nat. Genet. 27:392.
Hooi, C.F. et al. (2006) Oncogene 25:3924.
Sivasundaram, K. et al. (2003) Oncol. Rep. 10:1737.
Dong, S.M. and D. Sidransky (2002) Clin. Cancer Res. 8:2939.
Garnis, C. et al. (2004) Oncogene 23:2582.

Long Name

Low Density Lipoprotein Receptor-related 12

Entrez Gene IDs

29967 (Human); 239393 (Mouse)

Alternate Names

DKFZp762O2113; FAM4A1; LRP12; Protein FAM4A1; Protein HELG; RAY1; SEN4; ST7; subfamily A, member 1; suppression of tumorigenicity 7 (breast); suppression of tumorigenicity 7; suppressor of tumorigenicity 7 protein; TSG7