Human Syntaxin 16 Antibody

Catalog #: AF5648 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5648
AF5648-SP

Detection of Human Syntaxin 16 by Western Blot.

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All Syntaxin 16 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Syntaxin 16 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Syntaxin 16 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross‑reactivity with recombinant human (rh) Syntaxin 1A, rhSyntaxin 6, and rhSyntaxin BP1 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Syntaxin 16 isoform B
Leu165-Lys301
Accession # O14662

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Syntaxin 16 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Syntaxin 16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5648) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Syntaxin 16 at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Syntaxin 16

Syntaxin 16 (STX16; also SNY16) is a 39 kDa member of the syntaxin family of proteins. It is ubiquitously expressed, embedded in the Golgi membrane, and participates in the fusion of early endosomes with the Golgi stacks. Syntaxin 16 contributes one of four coiled-coil domains necessary for retrograde transport. Human Syntaxin 16 is a type IV single-pass transmembrane protein (very short lumenal C-terminus) that is 325 amino acids (aa) in length. It contains a cytoplasmic syntaxin region (aa 74‑180), a coiled-coil region (aa 230‑292), and a short, three amino acid C‑terminal lumenal sequence. There are multiple potential isoforms. Two show alternate start sites at Met187 and Met54, while three others show deletions of aa 45‑48, 28‑48 and 28‑44, respectively. A cytoplasmic form shows an Ala substitution for aa 132‑325. Over aa 165‑301, human Syntaxin 16 shares 95% aa identity with mouse Syntaxin 16.

Entrez Gene IDs

8675 (Human); 228960 (Mouse); 362283 (Rat)

Alternate Names

MGC90328; STX16; Syn16; SYN16hsyn16; Syntaxin 16; syntaxin-16