Human Syntaxin 16 Antibody Summary
Leu165-Lys301
Accession # O14662
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
![Detection of Human Syntaxin 16 antibody by Western Blot. Detection of Human Syntaxin 16 antibody by Western Blot.](https://resources.rndsystems.com/images/datasheets/antibody/Syntaxin_16_AF5648_Western_Blot_13648.jpg)
Detection of Human Syntaxin 16 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Syntaxin 16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5648) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Syntaxin 16 at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Syntaxin 16
Syntaxin 16 (STX16; also SNY16) is a 39 kDa member of the syntaxin family of proteins. It is ubiquitously expressed, embedded in the Golgi membrane, and participates in the fusion of early endosomes with the Golgi stacks. Syntaxin 16 contributes one of four coiled-coil domains necessary for retrograde transport. Human Syntaxin 16 is a type IV single-pass transmembrane protein (very short lumenal C-terminus) that is 325 amino acids (aa) in length. It contains a cytoplasmic syntaxin region (aa 74‑180), a coiled-coil region (aa 230‑292), and a short, three amino acid C‑terminal lumenal sequence. There are multiple potential isoforms. Two show alternate start sites at Met187 and Met54, while three others show deletions of aa 45‑48, 28‑48 and 28‑44, respectively. A cytoplasmic form shows an Ala substitution for aa 132‑325. Over aa 165‑301, human Syntaxin 16 shares 95% aa identity with mouse Syntaxin 16.
Product Datasheets
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