Human Syntaxin-BP2 Antibody

Catalog #: AF5900 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5900
AF5900-SP

Detection of Human Syntaxin-BP2 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Syntaxin-BP2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Syntaxin‑BP2 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross‑reactivity with recombinant human (rh) Syntaxin-BP1 and rhSyntaxin-BP3 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Syntaxin‑BP2
Glu81-Thr223
Accession # NP_001120868

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Syntaxin-BP2 by Western Blot. Western blot shows lysates of human peripheral blood leukocyte. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Syntaxin-BP2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5900) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Syntaxin-BP2 at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Syntaxin-BP2

Syntaxin‑BP2 (STXBP2; Syntaxin-binding protein 2; also Unc18-2 and Unc18B) is a 64‑66 kDa member of the SNARE-interacting Sec1/Unc18/STXBP family of proteins. It is widely expressed, particularly in hematopoietic cells, and is found both in cytoplasm, and associated with membranes, where it binds to STX1A, 2, and 3. Thus, it presumably participates in primary granule exocytosis by promoting granule fusion. Human STXBP2 is 593 amino acids (aa) in length. It contains one SEC1 domain (aa 28‑576) with Trp28, Ser42, and Glu59 all playing a key role in syntaxin binding. There is one splice variant that shows a deletion of aa 83‑85. Over aa 80‑223, human and mouse STXBP2 share 95% aa sequence identity.

Long Name

Syntaxin-binding Protein 2

Entrez Gene IDs

6813 (Human); 20911 (Mouse); 81804 (Rat)

Alternate Names

FHL5; Hunc18b; MUNC18-2; Protein unc-18 homolog 2; Protein unc-18 homolog B; STXBP2; syntaxin binding protein 2; syntaxin-binding protein 2; SyntaxinBP2; Syntaxin-BP2; Unc18-2; UNC18B; unc-18B; UNC18Bpp10122