Human T-bet/TBX21 PerCP-conjugated Antibody

Catalog #: IC5385C
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
IC5385C
IC5385C-025

Detection of T‑bet/TBX21 in Jurkat Human Cell Line by Flow Cytometry.

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All T-bet/TBX21 Antibodies

Product Details

Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human T-bet/TBX21 PerCP-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human T-bet.

Source

Monoclonal Mouse IgG₁ Clone # 525803

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human T-bet
Glu326-Asn535
Accession # Q9UL17

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

PerCP (Peridinin-chlorophyll Protein Complex) (Excitation= 482 and 564 nm, Emission= 675 nm)

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of T-bet/TBX21 antibody in Jurkat Human Cell Line antibody by Flow Cytometry.

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Detection of T‑bet/TBX21 in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human T-bet/TBX21 PerCP-conjugated Monoclonal Antibody (Catalog # IC5385C, filled histogram) or isotype control antibody (Catalog # IC002C, open histogram). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: T-bet/TBX21

T-box expressed in T cells (T-bet), also known as T-box transcription factor TBX21, is a 62 kDa member of the T-box family of transcription factors and the Tbr1 subfamily. Human T-bet is 535 amino acids in length and contains a T-box DNA binding domain (aa 136-327). Human T-bet shares 88% aa sequence identity with mouse T-bet. T-bet is a nuclear protein highly apparent in Th1-cells. Northern blot analysis revealed that it is also expressed in lung, thymus and spleen. Functionally, T-bet controls the expression of the Th1 cytokine, IFN gamma, and initiates Th1 lineage development from naïve Th precursor cells by both activating Th1 genetic programs and by repressing the opposing Th2 programs.

Long Name

T-box transcription factor TBX21

Entrez Gene IDs

30009 (Human); 57765 (Mouse); 303496 (Rat)

Alternate Names

Tbet; T-bet; TBETT-PET; TBLYM; TBLYMT-cell-specific T-box transcription factor T-bet; T-box 21; T-box expressed in T cells; T-box protein 21; T-box transcription factor TBX21; TBX21; T-PET; Transcription factor TBLYM

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human T-bet/TBX21 PerCP-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Lipopolysaccharide-Induced Chorioamnionitis Promotes IL-1-Dependent Inflammatory FOXP3+ CD4+ T Cells in the Fetal Rhesus Macaque
Authors: CM Rueda, P Presicce, CM Jackson, LA Miller, SG Kallapur, AH Jobe, CA Chougnet
J Immunol, 2016-04-01;0(0):.
Species: Primate - Macaca mulatta (Rhesus Macaque)
Sample Types: Whole Cells
Applications: Flow Cytometry