Human TACE/ADAM17 Cytosolic Domain Antibody Summary
Asp695-Cys824
Accession # P78536
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human TACE/ADAM17 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, Raji human Burkitt's lymphoma cell line, and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TACE/ADAM17 Cytosolic Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2129) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TACE/ADAM17 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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TACE/ADAM17 in Human Kidney Cancer Tissue. TACE/ADAM17 was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using Goat Anti-Human TACE/ADAM17 Cytosolic Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2129) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Detection of Human TACE/ADAM17 by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma parental cell line and TACE/ADAM17 knockout HeLa cell line, loaded at 0.2 mg/mL. A specific band was detected for TACE/ADAM17 at approximately 180 kDa (as indicated) in the HeLa parental cell line using 10 µg/mL of Goat Anti-Human TACE/ADAM17 Cytosolic Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2129) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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Western Blot Shows Human TACE/ADAM17 Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and TACE/ADAM17 knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TACE/ADAM17 Cytosolic Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2129) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TACE/ADAM17 at approximately 140 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TACE/ADAM17
TACE is a member of the ADAM family that contains A Disintegrin And Metalloprotease-like domain. Like other membrane-anchored ADAMs, TACE consists of a pro domain with a cysteine switch and furin cleavage sequence, a catalytic domain with the zinc-binding site and Met-turn expected for reprolysins, a disintegrin-like domain, a cysteine-rich domain, an EGF-like domain, a transmembrane domain, and the cytoplasmic domain. In addition to its ability to release the 17 kDa extracellular form of tumor necrosis factor-alpha (TNF-alpha ) from the 26 kDa membrane-anchored TNF-alpha, TACE also plays an essential role in shedding ectodomains from a variety of proteins such as L-Selectin, Transforming Growth Factor-alpha, Amyloid Protein Precursor, and Notch-1 receptor. TACE mRNA is present in virtually every tissue and TACE protein resides both on the cell surface and in the cell.
Product Datasheets
Citation for Human TACE/ADAM17 Cytosolic Domain Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Physical and functional interaction between the alpha- and gamma-secretases: A new model of regulated intramembrane proteolysis.
Authors: Chen A, Kim S, Shepardson N, Patel S, Hong S, Selkoe D
J Cell Biol, 2015-12-21;211(6):1157-76.
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