Human TACE/ADAM17 Ectodomain Antibody

Name: Human TACE/ADAM17 Ectodomain Antibody
Brand: R&D Systems
SKU: AF930
Rating: 5 (1 reviews)

Catalog #: AF930
4 Citations 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF930
AF930-SP

All TACE/ADAM17 Antibodies

Product Details

Citations (4)

FAQs

Supplemental Products

Reviews (1)

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TACE/ADAM17 Ectodomain Antibody Summary

Species Reactivity

Human

Specificity

Detects human TACE/ADAM17 Ectodomain in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross‑reactivity with recombinant human (rh) BACE, rhADAM8, rhADAM10, and recombinant mouse ADAM10 is observed.

Source

Polyclonal Chicken IgY

Purification

Antigen Affinity-purified from egg yolks

Immunogen

Insect ovarian cell line T. ni-derived recombinant human TACE/ADAM17
Pro18-Asn671
Accession # P78536

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human TACE/ADAM17 Western Blot Standard (Catalog # WBC029)

Immunohistochemistry

5-15 µg/mL

Immersion fixed paraffin-embedded sections of human prostate cancer tissue

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TACE/ADAM17

TACE is a member of the ADAM family that contains A Disintegrin And Metalloprotease-like domain. Like other membrane-anchored ADAMs, TACE consists of a pro domain with a cysteine switch and furin cleavage sequence, a catalytic domain with the zinc-binding site and Met-turn expected for reprolysins, a disintegrin-like domain, a cysteine-rich domain, an EGF-like domain, a transmembrane domain, and the cytoplasmic domain. In addition to its ability to release the 17 kDa extracellular form of tumor necrosis factor-alpha (TNF-alpha ) from the 26 kDa membrane-anchored TNF-alpha, TACE also plays an essential role in shedding ectodomains from a variety of proteins such as L-Selectin, Transforming Growth Factor-alpha, Amyloid Protein Precursor, and Notch-1 receptor. TACE mRNA is present in virtually every tissue and TACE protein resides both on the cell surface and in the cell.

References

Black, R.A. and J.D. Becherer (1998) in Tumor Necrosis Factor alpha -Converting Enzyme. Barrett, A.J. et al. (eds): Handbook of Proteolytic Enzymes, San Diego: Academic Press, p. 1315.
Primakoff, P. and D.G. Myles (2000) Trends in Genetics 16:83.

Long Name

TNF-alpha Converting Enzyme

Entrez Gene IDs

6868 (Human); 11491 (Mouse)

Alternate Names

ADAM 17; ADAM metallopeptidase domain 17; ADAM metallopeptidase domain 18; ADAM17; ADAM18; CD156b antigen; CD156b; CSVP; disintegrin and metalloproteinase domain-containing protein 17; EC 3.4.24.86; MGC71942; Snake venom-like protease; TACE; TACEcSVP; TNF-alpha convertase; TNF-alpha converting enzyme; TNF-alpha-converting enzyme; tumor necrosis factor, alpha, converting enzyme

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human TACE/ADAM17 Ectodomain Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Quantitative proteomics of MDCK cells identify unrecognized roles of clathrin adaptor AP-1 in polarized distribution of surface proteins
Authors: PS Caceres, D Gravotta, PJ Zager, N Dephoure, E Rodriguez-
Proc. Natl. Acad. Sci. U.S.A., 2019-05-29;116(24):11796-11805.
Species: Canine
Sample Types: Cell Lysates
Applications: Western Blot
The shedding activity of ADAM17 is sequestered in lipid rafts.
Authors: Tellier E, Rebsomen L
Exp. Cell Res., 2006-09-05;312(20):3969-80.
Species: Human, Mouse, Primate - Cholrocebus pygerythrus (Vervet Monkey)
Sample Types: Cell Lysates, Tissue Homogenates
Applications: Western Blot
FHL2 interacts with both ADAM-17 and the cytoskeleton and regulates ADAM-17 localization and activity.
Authors: Canault M, Tellier E, Bonardo B, Mas E, Aumailley M, Juhan-Vague I, Nalbone G, Peiretti F
J. Cell. Physiol., 2006-08-01;208(2):363-72.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation
ADAMs, a disintegrin and metalloproteinases, mediate shedding of oxytocinase.
Authors: Ito N, Nomura S, Iwase A, Ito T, Kikkawa F, Tsujimoto M, Ishiura S, Mizutani S
Biochem. Biophys. Res. Commun., 2004-02-20;314(4):1008-13.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot