Human TAFA3/FAM19A3 Antibody

Catalog #: MAB4677 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4677
MAB4677-SP

Detection of TAFA3/FAM19A3 in A172 Human Cell Line by Flow Cytometry.

1 Image

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TAFA3/FAM19A3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human TAFA3/FAM19A3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human TAFA1, 2, 4, or 5 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 460904

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human TAFA3/FAM19A3
Ala31-Arg133
Accession # Q7Z5A8

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human TAFA3/FAM19A3 under non-reducing conditions only

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of TAFA3/FAM19A3 antibody in A172 Human Cell Line antibody by Flow Cytometry.

View Larger

Detection of TAFA3/FAM19A3 in A172 Human Cell Line by Flow Cytometry. A172 human glioblastoma cell line was stained with Mouse Anti-Human TAFA3/FAM19A3 Monoclonal Antibody (Catalog # MAB4677, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TAFA3/FAM19A3

TAFA3 (also FAM19A3) is an 11 kDa (predicted) member of the FAM19/TAFA family of 5 proteins that show differential expression in the brain. It is probably a secreted protein. TAFA 1‑4 proteins bear chemokine-like signatures, and are distantly related to MIP-1 alpha, sharing its CC motif. A conserved 10 cysteine pattern suggests multiple disulfide linkages. Mature human TAFA3 is 103 aa in length and has no predicted N‑linked glycosylation sites. It shares 86% aa identity with mouse TAFA3. A potential 131 aa isoform diverges at aa 59 of the mature sequence.

Long Name

Family with Sequence Similarity 19, Member A3

Entrez Gene IDs

284467 (Human); 329731 (Mouse)

Alternate Names

Chemokine-like protein TAFA-3; FAM19A3; family with sequence similarity 19 (chemokine (C-C motif)-like), member A3; MGC138473; TAFA3; TAFA-3