Human TBX18 Antibody

Catalog #: MAB63371
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB63371
MAB63371-SP

2 Images

All TBX18 Antibodies

Product Details

Citations (1)

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TBX18 Antibody Summary

Species Reactivity

Human

Specificity

Detects human TBX18 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) TBX2, 3, 5, 6, 20, or rhT-bet is observed.

Source

Monoclonal Mouse IgG_2B Clone # 635305

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human TBX18
Ala384-Ser504
Accession # O95935

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

View Larger

TBX18 in Saos‑2 Human Cell Line. TBX18 was detected in immersion fixed Saos-2 human osteosarcoma cell line using Mouse Anti-Human TBX18 Monoclonal Antibody (Catalog # MAB63371) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry

View Larger

TBX18 in A549 Human Cell Line. TBX18 was detected in immersion fixed A549 human lung carcinoma cell line (positive staining) and MCF‑7 human breast cancer cell line using Mouse Anti-Human TBX18 Monoclonal Antibody (Catalog # MAB63371) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cell nuclei. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TBX18

TBX18 is a 607 amino acid, 65 kDa (predicted) protein that is a member of the T-box family of developmentally expressed transcription factors. It is developmentally expressed in the anterior portion of the head, somites and limb buds. It is expressed on pluripotent progenitors in the developing epicardium where it is required for formation of the cardiac AV node, and can also contribute to fibroblast and smooth muscle formation. Within the region used as an immunogen, human TBX18 shares 93% amino acid identity with mouse and rat TBX18.

Long Name

T-box Transcription Factor 18

Entrez Gene IDs

9096 (Human); 76365 (Mouse); 315870 (Rat)

Alternate Names

T-box 18; T-box protein 18; T-box transcription factor TBX18; TBX18

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human TBX18 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Co-emergence of cardiac and gut tissues promotes cardiomyocyte maturation within human iPSC-derived organoids
Authors: AC Silva, OB Matthys, DA Joy, MA Kauss, V Natarajan, MH Lai, D Turaga, AP Blair, M Alexanian, BG Bruneau, TC McDevitt
Cell Stem Cell, 2021-12-02;28(12):2137-2152.e6.
Species: Human
Sample Types: Organoid
Applications: IHC