Human TCF-3/E2A Antibody

Catalog #: MAB44062 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB44062
MAB44062-SP

Detection of Human TCF-3/E2A by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TCF-3/E2A Antibody Summary

Species Reactivity

Human

Specificity

Detects human TCF-3/E2A in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) TCF-1 (aa 116‑233), rhTCF-1 (aa 217-358), rhTCF-1 alpha/LEF-1, rhTCF-2, rhTCF-4, rhTCF-12, rhTCF7L1 (aa 151-320), rhTCFL1 (aa 429-581), or rhTCF7L2 (aa 131-300) is observed.

Source

Monoclonal Rat IgG_2A Clone # 401703

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E.coli-derived recombinant human TCF-3/E2A
Gln100-Glu500
Accession # NP_003191

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human TCF-3/E2A by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF Membrane was probed with 2 µg/mL of Human TCF-3/E2A Monoclonal Antibody (Catalog # MAB44062) overnight at 4^oC followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for TCF-3/E2A at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TCF-3/E2A

TCF-3, also known as E2A and ITF-1, is a member of the E protein homeobox family. TCF-3 regulates checkpoints that allow B lineage commitment and B and T cell receptor gene rearrangement. Two isoforms named E12 and E47 are alternatively spliced within the DNA binding and HLH domain. The region of human TCF-3 used as the immunogen, which is identical for E12 and E47, shares 80% and 79% aa identity with mouse and rat TCF-3, respectively.

Long Name

Transcription Factor 3/E2-alpha

Entrez Gene IDs

6929 (Human); 21423 (Mouse); 312451 (Rat)

Alternate Names

bHLHb21; bHLHb21TCF-3; Class B basic helix-loop-helix protein 21; E2A immunoglobulin enhancer-binding factor E12/E47; E2A; E2AKappa-E2-binding factor; Immunoglobulin enhancer-binding factor E12/E47; Immunoglobulin transcription factor 1; ITF1; ITF1Transcription factor ITF-1; Kappa-E2-binding factor; MGC129647; MGC129648; Pan2; TCF-3; Tcfe2a; transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47); Transcription factor 3; transcription factor E2-alpha; VDIR; VDR interacting repressor