Human TCF7L1 Antibody

Catalog #: MAB61161
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB61161
MAB61161-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TCF7L1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human TCF7L1 in direct ELISAs. In direct ELISAs, 5-10% cross-reactivity with recombinant human (rh) TCF-4 (aa 102-516) and rhTCF-12 is observed. No cross-reactivity with rhTCF7L1 (aa 151-320), rhTCF7L2, rhTCF-1, -2, -3, -4 (aa 241-365), rhLEF-1, or recombinant mouse TCF-1 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 622782

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E.coli-derived recombinant human TCF7L1
Lys429-Ser581
Accession # Q9HCS4

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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TCF7L1 in Human Brain. TCF7L1 was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Human TCF7L1 Monoclonal Antibody (Catalog # MAB61161) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TCF7L1/TCF3

Transcription factor 7-like 1 (TCF7L1; also HMG box transcription factor 3, TCF-3) is a 63 kDa member of the TCF/LEF family of proteins. Human TCF7L1 is 588 amino acids (aa) in length and contains one HMG box DNA-binding domain (aa 346-414). Human TCF7L1 shares 95% aa sequence identity with mouse TCF7L1. TCF7L1 is expressed in hair follicles and skin keratinocytes and at lower levels in stomach epithelium. Functionally, it participates in the Wnt signaling pathway. TCF7L1 binds to DNA and acts as a repressor in the absence of CTNNB1 and as an activator in its presence. TCF7L1 is also necessary for the terminal differentiation of epidermal cells, the formation of keratohyalin granules, and the development of the barrier function of the epidermis.

Long Name

Transcription Factor 7-like 1

Entrez Gene IDs

83439 (Human); 21415 (Mouse); 312451 (Rat)

Alternate Names

HMG box transcription factor 3; TCF3; TCF-3; TCF7L1; transcription factor 7-like 1 (T-cell specific, HMG-box); transcription factor 7-like 1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human TCF7L1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1