Human TFPI-2 Antibody

Catalog #: MAB25451
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB25451
MAB25451-SP

All TFPI-2 Antibodies

Product Details

Citations (1)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TFPI-2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human TFPI‑2 in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 243227

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TFPI‑2
Asp23-Phe235
Accession # P48307

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human TFPI‑2 (Catalog # 2545‑PI), see our available Western blot detection antibodies

Neutralization

Measured by its ability to neutralize Recombinant Human TFPI-2 (0.017 µg/mL, Catalog # 2545-PI) inhibition of Trypsin (0.025 µg/mL) cleavage of the fluorogenic peptide substrate Mca-RPKPVE-Nval-WRK(Dnp)-NH₂ (10 µM, Catalog # ES002). The Neutralization Dose (ND₅₀) is typically 0.45 µg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TFPI-2

Human Tissue Factor Pathway Inhibitor 2 (TFPI-2), also known as placental protein 5 (PP5) and retinal pigment epithelial cell factor 1 (REF-1), is a secreted protein with a N-terminal acidic region, three Kunitz (K) domains (residues 36 to 86, 96 to 149 and 158 to 208) separated with by two linker regions, and a C-terminal basic region (1‑3). Expression of TFPI-2 is down-regulated in several cancers, which may contribute to tumor progression in these cancers (4). The purified Recombinant Human TFPI-2 ends at residue 213 and does not contain the last 22 residues (residues 214 to 235) in the C-terminal region. It inhibits the activity of Recombinant Human Coagulation Factor VII (Catalog # 2338-SE) in the presence of Recombinant Human Tissue Factor (Catalog # 2339-PA).

References

Miyagi, Y. et al. (1994) J. Biochem. 116:939.
Sprecher, C.A. et al. (1994) Proc. Natl. Acad. Sci. USA 91:3353.
Tanaka, Y. et al. (2004) Invest. Ophthalmol. Vis. Sci. 45:245.
Rollin, J. et al. (2005) Br, J. Cancer 92:775.

Long Name

Tissue Factor Pathway Inhibitor 2

Entrez Gene IDs

7980 (Human)

Alternate Names

Placental protein 5; PP5; PP5FLJ21164; REF-1; TFPI2; TFPI-2; TFPI-2REF1; tissue factor pathway inhibitor 2

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human TFPI-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Syncytiotrophoblast microvesicles released from pre-eclampsia placentae exhibit increased tissue factor activity.
Authors: Gardiner C, Tannetta DS, Simms CA, Harrison P, Redman CW, Sargent IL
PLoS ONE, 2011-10-14;6(10):e26313.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization