Human Thrombopoietin R/Tpo R Antibody

Catalog #: AF1016
4 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF1016
AF1016-SP

Cell Proliferation Induced by Thrombopoietin/Tpo and Neutralization by Human Thrombopoietin R/Tpo R Antibody.

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Citations (4)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Thrombopoietin R/Tpo R Antibody Summary

Species Reactivity

Human

Specificity

Detects human Thrombopoietin R/Tpo R in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross‑reactivity with recombinant human Epo R is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Thrombopoietin R/Tpo R
Gln26-Tyr423
Accession # P40238

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Thrombopoietin R/Tpo R (Catalog # 4444-TR)

Neutralization

Measured by its ability to neutralize Thrombopoietin/Tpo-induced proliferation in the MO7e human megakaryocytic leukemic cell line. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359. The Neutralization Dose (ND₅₀) is typically 0.5‑2.5 µg/mL in the presence of 10 ng/mL Recombinant Human Thrombopoietin/Tpo.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Cell Proliferation Induced by Thrombopoietin/Tpo and Neutralization by Human Thrombopoietin R/Tpo R Antibody. Recombinant Human Thrombopoietin/Tpo (Catalog # 288-TP) stimulates proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Thrombopoietin/Tpo (10 ng/mL) is neutralized (green line) by increasing concen-trations of Goat Anti-Human Thrombopoietin R/Tpo R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1016). The ND₅₀ is typically 0.5-2.5 µg/mL.

Simple Western

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Detection of Human Thrombopoietin R/Tpo R by Simple Western^TM. Simple Western lane view shows lysates of Daudi human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Thrombopoietin R/Tpo R at approximately 73 kDa (as indicated) using 20 µg/mL of Goat Anti-Human Thrombopoietin R/Tpo R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Thrombopoietin R/Tpo R

Thrombopoietin receptor (Tpo R), also known as myeloproliferative leukemia protein (c-mpl), is a 95 kDa type I transmembrane protein that is a member of the type I cytokine receptor family within the hematopoietin/cytokine receptor superfamily (1‑4). The 635 amino acid (aa) full-length human Tpo R contains a 25 aa signal sequence, a 466 aa extracellular domain with a ligand binding domain and two fibronectin type III domains, a transmembrane (TM) domain and a cytoplasmic domain. The extracellular domain of human Tpo R shares 78%, 76%, 81%, 82% and 80% aa identity with mouse, rat, bovine, canine and equine Tpo R, respectively. Humans produce three distinct mRNA species; a P-form, a K-form, and a truncated form (Mpl-tr) lacking a TM domain (3‑7). The P-form encodes the full-length receptor. The Mpl-tr form, which is expressed in both human and mouse, is intracellular and targets the P-form for degradation (5, 6). The 579 aa K-form has an alternate cytoplasmic domain, but does not dimerize with, or inhibit, the P-form (7). Thrombopoietin (Tpo) is a key regulator of megakaryocytopoiesis, thrombopoiesis and hematopoietic stem cell self-renewal, as reflected by expression of the Tpo R on megakaryocytes, platelets and hematopoietic progenitors (2, 8). Receptor dimerization occurs upon Tpo binding and initiates signaling through the Ras/MAP and JAK/STAT pathways (1, 2). Internalization and degradation of Tpo following Tpo R binding serves to downregulate circulating Tpo (9). Tpo R expressed at low levels on endothelial cells does not appear to contribute to regulation of Tpo (10). Inactivating mutations of Tpo R cause thrombocytopenia, and absence of functional Tpo R is lethal in humans, but not mice. Other mutations, including an activating change in the TM domain, can cause thrombocytosis (11, 12).

References

Kaushansky, K. (2005) J. Clin. Invest. 115:3339.
Deutsch, V.R. and A. Tomer (2006) Br. J. Haematol. 134:453.
Vigon, I. et al. (1992) Proc. Natl. Acad. Sci. USA 89:5640.
Mignotte, V. et al. (1994) Genomics 20:5.
Li, J. et al. (2000) Cytokine 12:835.
Coers, J. et al. (2004) J. Biol. Chem. 279:36397.
Millot, G.A. et al. (2002) Exp. Hematol. 30:166.
Tong, W. et al. (2007) Exp. Hematol. July 14 [Epub ahead of print].
Li, J. et al. (1999) Br. J. Haematol. 106:345.
Geddis, A.E. et al. (2006) Exp. Hematol. 34:82.
Germeshausen, M. et al. (2006) Hum. Mutat. 27:296.
Ding, J. et al. (2004) Blood 103:4198.

Long Name

Thrombopoietin Receptor

Entrez Gene IDs

4352 (Human); 17480 (Mouse)

Alternate Names

CD110 antigen; CD110; c-Mpl; MPL; MPLV; Myeloproliferative leukemia protein; myeloproliferative leukemia virus oncogene; Proto-oncogene c-Mpl; Thrombopoietin R; thrombopoietin receptor; TpoR; TPO-R; TPORMPLV

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Citations for Human Thrombopoietin R/Tpo R Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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AC-73 and Syrosingopine Inhibit SARS-CoV-2 Entry into Megakaryocytes by Targeting CD147 and MCT4
Authors: Spinello, I;Saulle, E;Quaranta, MT;Pelosi, E;Castelli, G;Cerio, A;Pasquini, L;Morsilli, O;Dupuis, ML;Labbaye, C;
Viruses
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot
Thrombopoietin participates in platelet activation in COVID-19 patients
Authors: E Lupia, M Capuano, B Vizio, M Schiavello, O Bosco, M Gelardi, E Favale, E Pivetta, F Morello, S Husain, S Keshavjee, L Del Sorbo, G Montrucchi
EBioMedicine, 2022-10-13;85(0):104305.
Species: Human
Sample Types: Plasma
Applications: Neutralization
Increased Toxoplasma gondii Intracellular Proliferation in Human Extravillous Trophoblast Cells (HTR8/SVneo Line) Is Sequentially Triggered by MIF, ERK1/2, and COX-2
Authors: ICB Milian, RJ Silva, C Manzan-Mar, BF Barbosa, PM Guirelli, M Ribeiro, A de Oliveir, F Ietta, JR Mineo, P Silva Fran, EAV Ferro
Front Microbiol, 2019-04-24;10(0):852.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot
BMP4 regulation of human megakaryocytic differentiation is involved in thrombopoietin signaling.
Authors: Jeanpierre S, Nicolini FE, Kaniewski B, Dumontet C, Rimokh R, Puisieux A, Maguer-Satta V
Blood, 2008-07-29;112(8):3154-63.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization