Human Thrombospondin-2 Quantikine ELISA Kit Summary
Product Summary
Precision
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (ng/mL) | 4.08 | 7.73 | 10.9 | 4.11 | 7.71 | 11.4 |
| Standard Deviation | 0.189 | 0.229 | 0.281 | 0.295 | 0.511 | 0.726 |
| CV% | 4.6 | 3 | 2.6 | 7.2 | 6.6 | 6.4 |
Serum, EDTA Plasma, Heparin Plasma, Human Milk
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (ng/mL) | 3.97 | 7.96 | 11.4 | 4.12 | 7.68 | 11.4 |
| Standard Deviation | 0.198 | 0.205 | 0.284 | 0.259 | 0.403 | 0.527 |
| CV% | 5 | 2.6 | 2.5 | 6.3 | 5.2 | 4.6 |
Recovery
The recovery of Thrombospondin-2 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 101 | 91-108 |
| EDTA Plasma (n=4) | 93 | 86-105 |
| Heparin Plasma (n=4) | 92 | 86-103 |
| Serum (n=4) | 93 | 86-105 |
Linearity
Scientific Data
Preparation and Storage
Background: Thrombospondin-2
Thrombospondins (TSP) are secreted multidomain glycoproteins with many putative functions including modulating cell adhesion, proliferation, migration, and angiogenesis. At least five TSPs exist: TSP-1, -2, -3, -4, and cartilage oligomeric glycoprotein (COMP)/TSP-5. The family can be divided into two subgroups. The first, TSP-1 and -2, are homotrimeric molecules, each monomer containing an N-terminal/heparin-binding domain, a coiled-coil oligomerization domain, a von Willebrand factor-type/procollagen homology domain, three type 1 thrombospondin repeats (TSR), three EGF-like repeats, seven TSP type 3 repeats, and a C-terminal globular domain. TSP-1 and -2 are thought to mediate interactions between cells and the extracellular matrix, and as such are matricellular proteins. In contrast, TSP-3, -4, and COMP/TSP-5 are homopentamers that lack the N-terminal structure of TSP-1 and -2, but have a similar arrangement of EGF and TSP type 3 repeats, and the C-terminal domain characteristic of the TSP family.
Assay Procedure
Refer to the product- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 100 µL of Assay Diluent to each well.
- Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
- Aspirate and wash 4 times.
- Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
- Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations for Human Thrombospondin-2 Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 8
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Thrombospondin-2 as a Predictive Biomarker for Hepatocellular Carcinoma after Hepatitis C Virus Elimination by Direct-Acting Antiviral
Authors: T Matsumae, T Kodama, Y Tahata, Y Myojin, A Doi, A Nishio, R Yamada, Y Nozaki, M Oshita, N Hiramatsu, N Morishita, K Ohkawa, T Hijioka, M Sakakibara, Y Doi, N Kakita, T Yakushijin, R Sakamori, H Hikita, T Tatsumi, T Takehara
Cancers, 2023-01-11;15(2):.
Species: Human
Sample Types: Serum
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Circulating thrombospondin 2 levels reflect fibrosis severity and disease activity in HCV-infected patients
Authors: T Iwadare, T Kimura, N Tanaka, T Yamazaki, SI Wakabayash, T Okumura, H Kobayashi, Y Yamashita, SP Pydi, T Nakajima, M Iwaya, A Sugiura, S Joshita, T Uehara, T Umemura
Scientific Reports, 2022-11-07;12(1):18900.
Species: Human
Sample Types: Serum
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The circulating proteomic signature of alcohol-associated liver disease
Authors: J Luther, AG Vannier, EA Schaefer, RP Goodman
JCI Insight, 2022-07-22;7(14):.
Species: Human
Sample Types: Serum
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Pharmacological normalization of pancreatic cancer-associated fibroblast secretome impairs pro-metastatic cross-talk with macrophages: Stromal CSF-1 facilitates metastasis
Authors: R Samain, A Brunel, T Douché, M Fanjul, S Cassant-So, J Rochotte, J Cros, C Neuzillet, J Raffenne, C Duluc, A Perraud, J Nigri, V Gigoux, I Bieche, M Ponzo, G Carpentier, I Cascone, R Tomasini, HA Schmid, M Mathonnet, R Nicolle, MP Bousquet, Y Martineau, S Pyronnet, C Jean, C Bousquet
Cellular and Molecular Gastroenterology and Hepatology, 2021-01-20;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
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A Blood-Based Multi Marker Assay Supports the Differential Diagnosis of Early-Stage Pancreatic Cancer
Authors: AW Berger, D Schwerdel, A Reinacher-, W Uhl, H Algül, H Friess, KP Janssen, A König, M Ghadimi, E Gallmeier, DK Bartsch, M Geissler, L Staib, A Tannapfel, A Kleger, A Beutel, LA Schulte, M Kornmann, TJ Ettrich, T Seufferlei
Theranostics, 2019-02-12;9(5):1280-1287.
Species: Human
Sample Types: Plasma
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Thrombospondin-2 predicts response to treatment with intravenous immunoglobulin in children with Kawasaki disease
Authors: S Yang, R Song, X Li, T Zhang, J Fu, X Cui
BMJ Paediatr Open, 2018-01-03;2(1):e000190.
Species: Human
Sample Types: Plasma
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Circulating thrombospondin-2 reflects disease severity and predicts outcome of heart failure with reduced ejection fraction.
Authors: Hanatani S, Izumiya Y, Takashio S, Kimura Y, Araki S, Rokutanda T, Tsujita K, Yamamoto E, Tanaka T, Yamamuro M, Kojima S, Tayama S, Kaikita K, Hokimoto S, Ogawa H
Circ J, 2014-02-06;78(4):903-10.
Species: Human
Sample Types: Serum
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Tumor-derived pancreatic stellate cells promote pancreatic cancer cell invasion through release of thrombospondin-2.
Authors: Farrow B, Berger DH, Rowley D
J. Surg. Res., 2009-05-03;156(1):155-60.
Species: Human
Sample Types: Cell Culture Supernates
FAQs
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What is the normal appearance of Assay Diluent RD1-75?
Assay Diluent RD1-75 contains high concentrations of additives and typically has a very cloudy, brown appearance. It is recommended to mix this diluent well before and during use.
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