Human Thrombospondin-4 Antibody

Detection of Human Thrombospondin‑4 by Western Blot. Western blot shows lysates of human heart tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human Thrombospondin-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2390) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Thrombospondin-4 at approximately 130kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human Thrombospondin‑4 by Simple Western^TM. Simple Western lane view shows lysates of human heart tissue, loaded at 0.2 mg/mL. A specific band was detected for Thrombospondin-4 at approximately 158 kDa (as indicated) using 2 µg/mL of Goat Anti-Human Thrombospondin-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2390) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of Mouse Thrombospondin-4 by Immunocytochemistry/Immunofluorescence TSP-4 promotes accumulation of macrophages in peritoneal tissue of mice with LPS-induced peritonitis.a The number of macrophages in peritoneal cavity in mice with LPS-induced peritonitis. *p < 0.05, n = 5. b TSP-4 expression in macrophages from the peritoneal cavity lavage (left panel) and in the peritoneal tissue. QRT-PCR, fold increase (RQ) over the values in control mice injected with PBS; n = 3; *p < 0.05. c Macrophages and TSP-4 in peritoneal tissue of WT and P387-TSP-4-KI mice with LPS-induced peritonitis. Immunofluorescence; blue = nuclei (DAPI), green = macrophages (anti-CD68), red = TSP-4 (anti-TSP-4). Scale bar is 20 µm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31974349), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse Thrombospondin-4 by Immunocytochemistry/Immunofluorescence LPS induces TSP-4 in cultured macrophages.a Cultured macrophage-like RAW264.7 and cells and mouse bone-marrow-derived macrophages (BMDM) were treated with LPS (0.5 μg/ml for 1–24 h); western blotting with anti-TSP-4 Ab. b Cultured RAW264.7 (upper panels) and BMDM (lower panels) treated with LPS for 24 h were stained with anti-TSP-4 Ab; green = TSP-4, blue = nuclei, DAPI. Scale bar is 10 µm. c RAW 264.7 and BMDM cells were treated with LPS (0.5 μg/mL) for 24 h, and qRT-PCR was done (control = PBS treated); d RAW 264.7 cells were treated with CHX (25 μM) for 1–5 h and CHX (25 μM) + LPS (0.5 μg/mL) for 1–3 h followed by western blot detection of TSP-4 ( beta -actin as loading controls). CHX was added at time 0, followed by addition of LPS. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31974349), licensed under a CC-BY license. Not internally tested by R&D Systems.