Human TIMP-2 Antibody Summary
Cys27-Pro220
Accession # P16035
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human TIMP‑2 by Western Blot. Western blot shows Recombinant Human TIMP-2 Western Blot Standard Protein (Catalog # WBC023) and lysates of HeLa human cervical epithelial carcinoma cell line and human placenta tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human TIMP-2 Monoclonal Antibody (Catalog # MAB971) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for TIMP-2 at approximately 22 kDa (as indicated).This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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TIMP‑2 in Human Ovarian Cancer Tissue. TIMP-2 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using 15 µg/mL Mouse Anti-Human TIMP-2 Monoclonal Antibody (Catalog # MAB971) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS003) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TIMP-2
Tissue inhibitors of metalloproteinases or TIMPs are a family of proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs). There are four members of the family, TIMP-1, TIMP-2, TIMP-3, and TIMP-4. TIMP-2 is a non N-glycosylated protein with a molecular mass of 22 kDa produced by a wide range of cell types, which inhibits MMPs non-covalently by the formation of binary complexes. TIMP-2 also has erythroid-potentiating and cell growth promoting activities.
Product Datasheets
Citations for Human TIMP-2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
13
Citations: Showing 1 - 10
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PhosY-secretome profiling combined with kinase-substrate interaction screening defines active c-Src-driven extracellular signaling
Authors: Backe, SJ;Votra, SD;Stokes, MP;Sebesty�n, E;Castelli, M;Torielli, L;Colombo, G;Woodford, MR;Mollapour, M;Bourboulia, D;
Cell reports
Species: Human
Sample Types: Whole Cells
Applications: Immunoprecipitation -
Improved contractile potential in detrusor microtissues from pediatric patients with end stage lower urinary tract dysfunction
Authors: Gerwinn T, Salemi S, Schori LJ et al.
Frontiers in Cell and Developmental Biology
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Myosin Heavy Chain 10 (MYH10) Gene Silencing Reduces Cell Migration and Invasion in the Glioma Cell Lines U251, T98G, and SHG44 by Inhibiting the Wnt/ beta -Catenin Pathway
Authors: Yang Wang, Qi Yang, Yanli Cheng, Meng Gao, Lei Kuang, Chun Wang
Medical Science Monitor
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MicroRNA?122 acts as tumor suppressor by targeting TRIM29 and blocking the activity of PI3K/AKT signaling in nasopharyngeal carcinoma in�vitro
Authors: Y Yang, Q Li, L Guo
Mol Med Rep, 2018-04-19;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Carboxypeptidase E transmits its anti-migratory function in glioma cells via transcriptional regulation of cell architecture and motility regulating factors
Authors: Angela Armento, Elena I. Ilina, Tony Kaoma, Arnaud Muller, Laurent Vallar, Simone P. Niclou et al.
International Journal of Oncology
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Enhanced Immunoreactivity of TIMP-2 in the Stromal Compartment of Tumor as a Marker of Favorable Prognosis in Ovarian Cancer Patients
Authors: Agnieszka Hałoń, Ewa Nowak-Markwitz, Piotr Donizy, Rafał Matkowski, Adam Maciejczyk, Tserenchunt Gansukh et al.
Journal of Histochemistry & Cytochemistry
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Granule exocytosis is required for platelet spreading: differential sorting of alpha-granules expressing VAMP-7.
Blood, 2012-05-15;120(1):199-206.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Conditionally replicating adenovirus expressing TIMP2 for ovarian cancer therapy.
Authors: Yang SW, Cody JJ, Rivera AA, Waehler R, Wang M, Kimball KJ, Alvarez RA, Siegal GP, Douglas JT, Ponnazhagan S
Clin. Cancer Res., 2010-11-29;17(3):538-49.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
The DSCs-expressed CD82 controls the invasiveness of trophoblast cells via integrinbeta1/MAPK/MAPK3/1 signaling pathway in human first-trimester pregnancy.
Authors: Li MQ, Hou XF, Shao J, Tang CL, Li DJ
Biol. Reprod., 2010-01-14;82(5):968-79.
Species: Human
Sample Types: Whole Cells
Applications: Cell-based ELISA -
Tissue inhibitors of matrix metalloproteinases in platelets and megakaryocytes: a novel organization for these secreted proteins.
Authors: Villeneuve J, Block A, Le Bousse-Kerdiles MC, Lepreux S, Nurden P, Ripoche J, Nurden AT
Exp. Hematol., 2009-05-03;37(7):849-56.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Matrix metalloproteinase activity in pediatric acute lung injury.
Authors: Kong MY, Gaggar A, Li Y
Int J Med Sci, 2008-12-16;6(1):9-17.
Species: Human
Sample Types: Tissue Secretion
Applications: Western Blot -
IL-21 is highly produced in Helicobacter pylori-infected gastric mucosa and promotes gelatinases synthesis.
Authors: Caruso R, Fina D, Peluso I, Fantini MC, Tosti C, Del Vecchio Blanco G, Paoluzi OA, Caprioli F, Andrei F, Stolfi C, Romano M, Ricci V, MacDonald TT, Pallone F, Monteleone G
J. Immunol., 2007-05-01;178(9):5957-65.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot -
Control of matrix metalloproteinase production in human intestinal fibroblasts by interleukin 21.
Authors: Monteleone G, Caruso R, Fina D, Peluso I, Gioia V, Stolfi C, Fantini MC, Caprioli F, Tersigni R, Alessandroni L, MacDonald TT, Pallone F
Gut, 2006-05-08;55(12):1774-80.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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