Human TLR1 Antibody

Catalog #: AF1484 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF1484
AF1484-SP

All TLR1 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TLR1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human TLR1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 15% cross-reactivity with recombinant mouse (rm) TLR1 is observed and less than 5% cross-reactivity with recombinant human (rh) TLR3, rhTLR4, and rmTLR6 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TLR1
Ser22-Asn578
Accession # AAC34137

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.01 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human TLR1 Fc Chimera (Catalog # 1484-TR)

Flow Cytometry

0.25 µg/10⁶ cells

Human whole blood monocytes

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TLR1

The Toll-like family of molecules are type I transmembrane proteins that serve as pattern recognition receptors for microbial pathogens. There are at least eleven mouse and ten human TLRs that activate the innate immune system following exposure to a variety of microbial species (1, 2). TLRs contain a large number of leucine-rich repeats (LRRs) and a cytoplasmic tail with one Toll/IL-1 receptor (TIR) domain. Mature human TLR1 consists of a 556 amino acid (aa) extracellular domain (ECD) with 20 LRRs, a 21 aa transmembrane segment, and a 185 aa cytoplasmic domain (3, 4). Within the ECD, human TLR1 shares 63% aa sequence identity with human TLR6 and 20%‑43% aa sequence identitity with human TLR2, -3, -4, -5, -7, -8, -9, and -10. It shares 73% and 71% aa sequence identity with mouse and rat TLR1, respectively. TLR1 is expressed on the surface of macrophages, dendritic cells, and tonsillar epithelial cells in ligand-independent association with TLR2 (5‑8). TLR2 additionally associates with TLR6 to form a functional complex with specificity for distinct but related microbial ligands (9‑11). TLR1 and TLR2 cooperate in the recognition of bacterial and protozoal triacylated lipopeptides and glycosylphosphatidylinositols (6, 10‑12). Ligand binding induces TLR1 localization to lipid rafts followed by receptor internalization and activation of NF kappa B (7, 11, 13).

References

Miyake, K. (2007) Semin. Immunol. 19:3.
Hopkins, P.A. and S. Sriskandan (2005) Clin. Exp. Immunol. 140:395.
Rock, F.L. et al. (1998) Proc. Natl. Acad. Sci. 95:588.
Matsushima, N. et al. (2007) BMC Genomics 8:124.
Ochoa, M.-T. et al. (2003) Immunology 108:10.
Takeuchi, O. et al. (2002) J. Immunol. 169:10.
Triantafilou, M. et al. (2006) J. Biol. Chem. 281:31002.
Sandor, F. et al. (2003) J. Cell Biol. 162:1099.
Nakao, Y. et al. (2005) J. Immunol. 174:1566.
Ozinsky, A. et al. (2000) Proc. Natl. Acad. Sci. 97:13766.
Lee, J.Y. et al. (2004) J. Biol. Chem. 279:16971.
Krishnegowda, G. et al. (2005) J. Biol. Chem. 280:8606.
Nishiya, T. and A.L. DeFranco (2004) J. Biol. Chem. 279:19008.

Long Name

Toll-like Receptor 1

Entrez Gene IDs

7096 (Human); 21897 (Mouse); 305354 (Rat)

Alternate Names

CD281 antigen; CD281; DKFZp547I0610; DKFZp564I0682; KIAA0012MGC126311; MGC104956; MGC126312; rsc786; TIL; TIL. LPRS5; TLR1; Toll/interleukin-1 receptor-like protein; toll-like receptor 1