Human TLR2 Antibody

Name: Human TLR2 Antibody
Brand: R&D Systems
SKU: MAB2616
Rating: 4.7 (3 reviews)

Catalog #: MAB2616
17 Citations 3 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2616
MAB2616-SP

IL‑8 Secretion Induced by Pam3CSK4and Neutralization by Human TLR2 Antibody.

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All TLR2 Antibodies

Product Details

Citations (17)

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Supplemental Products

Reviews (3)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TLR2 Antibody Summary

Species Reactivity

Human

Specificity

Stains human TLR2 transfectants but not irrelevant transfectants.

Source

Monoclonal Mouse IgG_2B Clone # 383936

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human TLR2
Accession # O60603

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Flow Cytometry

2.5 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Neutralization

Measured by its ability to neutralize Pam₃CSK₄-induced IL‑8 secretion in the HEK293 human embryonic kidney cell line transfected with human TLR2. The Neutralization Dose (ND₅₀) is typically 0.03-0.15 µg/mL in the presence of 0.5 µg/mL The synthetic tripalmitoylated lipopeptide Pam₃CSK₄.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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IL‑8 Secretion Induced by Pam₃CSK₄and Neutralization by Human TLR2 Antibody. The synthetic tripalmitoylated lipopeptide Pam₃CSK₄stimulates IL-8 secretion in the HEK293 human embryonic kidney cell line transfected with human TLR2, in a dose-dependent manner (orange line), as measured by the Human CXCL8/IL-8 Quantikine ELISA Kit (Catalog # D8000C). IL-8 secretion elicited by Pam₃CSK₄(0.5 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human TLR2 Monoclonal Antibody (Catalog # MAB2616). The ND₅₀ is typically 0.03-0.15 µg/mL.

Flow Cytometry

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Detection of TLR2 in Human Monocytes by Flow Cytometry. Human whole blood monocytes were stained with Mouse Anti-Human TLR2 Monoclonal Antibody (Catalog # MAB2616, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TLR2

Human toll-like receptor (TLR) family includes ten members that activate the innate immune response via an ability to recognize molecular structures found in a variety of microbial pathogens (1‑3). All TLR family members are type I transmembrane proteins with a large number of extracellular leucine-rich repeats (LRRs) and a cytoplasmic Toll/IL-1 receptor (TIR) domain. Human TLR2 is synthesized as a 784 amino acid (aa) precursor (2) that contains a signal sequence (aa 1-18), an extracellular domain (aa 19-588) with approximately 20 LRRs, a transmembrane segment (aa 589-609), and a cytoplasmic TIR domain (aa 610-784). The receptor is expressed on a number of cell types including monocytes, dendritic cells, neutrophils, B cells endothelial cells, and hepatocytes (1, 2, 4). TLR2 functions as part of a heterodimeric complex with either TLR1 or TLR6, and possibly other co-receptors (1). These complexes recognize lipoproteins and glycolipids from gram-positive and gram-negative bacteria as well as mycoplasma and yeast. TLR2/TLR1 heterodimers bind triacylated lipopeptides, while the TLR2/TLR6 heterodimer preferentially recognizes diacylated lipopeptides (5). Upon ligand recognition, TLR2 delivers an activating signal via the associated adapter molecules, MyD88 and TIRAP (1, 6). TLR2 signaling results in dendritic cell maturation characterized by increased surface expression of class II MHC and the T cell costimulators, CD80 and CD86 (1, 2). Activation via TLR2 also results in production of a number of pro-inflammatory cytokines including TNF-alpha, IL-2, IL-6, IL-12, and MIP-2 (1-3).

References

Wetzler, L. (2003) Vaccine 21:S2/55.
Kirschning, C. and R. Schumann (2002) Curr. Top. microbiol. Immunol. 270:121.
Netea, M. et al. (2004) J. Leukoc. Biol. 75:749.
Flo, T. et al. (2001) J. Leukoc. Biol. 69:474.
Akira, S. (2003) Curr. Opin. Immunol. 15:5.
Yamamoto, M. et al. (2002) Nature 420:324.

Long Name

Toll-like Receptor 2

Entrez Gene IDs

7097 (Human); 24088 (Mouse); 310553 (Rat)

Alternate Names

CD282 antigen; CD282; TIL4CD282; TLR2; toll/interleukin 1 receptor-like 4; Toll/interleukin-1 receptor-like protein 4; toll-like receptor 2

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human TLR2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
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Role of TLR4 Receptor Complex in the Regulation of the Innate Immune Response by Fibronectin
Authors: M Zheng, A Ambesi, P J McKeown-
Cells, 2020-01-15;9(1):.
Applications: Neutralization
CLEC5A is a critical receptor in innate immunity against Listeria infection
Authors: ST Chen, FJ Li, TY Hsu, SM Liang, YC Yeh, WY Liao, TY Chou, NJ Chen, M Hsiao, WB Yang, SL Hsieh
Nat Commun, 2017-08-21;8(1):299.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
Dendritic Cell Sensing of Hydrophobic Di- and Triacylated Lipopeptides Self-Assembled within Synthetic Virus-like Particles
Authors: R Sharma, A Ghasparian, JA Robinson, KC McCullough
J. Immunol., 2017-06-19;0(0):.
Species: Porcine
Sample Types: Whole Cells
Applications: Functional Assay
Specific pathways mediating inflammasome activation by Candida parapsilosis
Authors: A Tóth, E Zajta, K Csonka, C Vágvölgyi, MG Netea, A Gácser
Sci Rep, 2017-02-22;7(0):43129.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
Kluyveromyces marxianus and Saccharomyces boulardii Induce Distinct Levels of Dendritic Cell Cytokine Secretion and Significantly Different T Cell Responses In Vitro
PLoS ONE, 2016-11-29;11(11):e0167410.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
A Model to Explain How the Bacille Calmette Guï¿½rin (BCG) Vaccine Drives Interleukin-12 Production in Neonates
PLoS ONE, 2016-08-29;11(8):e0162148.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Vaccine-Mediated Activation of Human TLR4 Is Affected by Modulation of Culture Conditions during Whole-Cell Pertussis Vaccine Preparation
PLoS ONE, 2016-08-22;11(8):e0161428.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
TLR2 Activation Limits Rhinovirus-Stimulated CXCL-10 by Attenuating IRAK-1-Dependent IL-33 Receptor Signaling in Human Bronchial Epithelial Cells
J Immunol, 2016-08-08;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
HMGB1 induces angiogenesis in rheumatoid arthritis via HIF-1alpha activation.
Authors: Park S, Lee S, Kim H, Lee W, Hong K, Kim C
Eur J Immunol, 2015-01-15;45(4):1216-27.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P
Necrotic cell-derived high mobility group box 1 attracts antigen-presenting cells but inhibits hepatocyte growth factor-mediated tropism of mesenchymal stem cells for apoptotic cell death.
Authors: Vogel S, Borger V, Peters C, Forster M, Liebfried P, Metzger K, Meisel R, Daubener W, Trapp T, Fischer J, Gawaz M, Sorg R
Cell Death Differ, 2015-01-09;22(7):1219-30.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization

Bordetella pertussis naturally occurring isolates with altered lipooligosaccharide structure fail to fully mature human dendritic cells.
Authors: Brummelman J, Veerman R, Hamstra H, Deuss A, Schuijt T, Sloots A, Kuipers B, van Els C, van der Ley P, Mooi F, Han W, Pinelli E
Infect Immun, 2014-10-27;83(1):227-38.
Species: Human
Sample Types: Whole Cells
Applications: Blocking

Activated platelets interfere with recruitment of mesenchymal stem cells to apoptotic cardiac cells via high mobility group box 1/Toll-like receptor 4-mediated down-regulation of hepatocyte growth factor receptor MET.
Authors: Vogel S, Chatterjee M, Metzger K, Borst O, Geisler T, Seizer P, Muller I, Mack A, Schumann S, Buhring H, Lang F, Sorg R, Langer H, Gawaz M
J Biol Chem, 2014-02-24;289(16):11068-82.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry, Neutralization

Mycobacteria bypass mucosal NF-kB signalling to induce an epithelial anti-inflammatory IL-22 and IL-10 response.
Authors: Lutay N, Hakansson G, Alaridah N, Hallgren O, Westergren-Thorsson G, Godaly G
PLoS ONE, 2014-01-28;9(1):e86466.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Tumor-produced versican V1 enhances hCAP18/LL-37 expression in macrophages through activation of TLR2 and vitamin D3 signaling to promote ovarian cancer progression in vitro.
Authors: Li D, Wang X, Wu J, Quan W, Ma L, Yang F, Wu K, Wan H
PLoS ONE, 2013-02-12;8(2):e56616.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Mycobacterium bovis bacilli Calmette-Guerin regulates leukocyte recruitment by modulating alveolar inflammatory responses.
Authors: Andersson M, Lutay N, Hallgren O, Westergren-Thorsson G, Svensson M, Godaly G
Innate Immun, 2011-11-04;18(3):531-40.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: Flow Cytometry, ICC, Neutralization, Western Blot

Isotretinoin increases skin-surface levels of neutrophil gelatinase-associated lipocalin in patients treated for severe acne.
Authors: Lumsden KR, Nelson AM, Dispenza MC, Gilliland KL, Cong Z, Zaenglein AL, Thiboutot DM
Br. J. Dermatol., 2011-08-01;165(2):302-10.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Soluble forms of Toll-like receptor 4 are present in human saliva and modulate tumour necrosis factor-alpha secretion by macrophage-like cells.
Authors: Zunt SL, Burton LV, Goldblatt LI, Dobbins EE, Srinivasan M
Clin. Exp. Immunol., 2009-03-09;156(2):285-93.
Species: Human
Sample Types: Saliva
Applications: ELISA Development