Human TLR4 Antibody

Name: Human TLR4 Antibody
Brand: R&D Systems
SKU: MAB6248
Rating: 5 (1 reviews)

Catalog #: MAB6248 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6248
MAB6248-SP

Detection of TLR4 in Human PBMC by Flow Cytometry.

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All TLR4 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TLR4 Antibody Summary

Species Reactivity

Human

Specificity

Detects human TLR4 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) TLR1, 2, 3, 5, 7, 8, 9, 10, recombinant mouse (rm) TLR4, rmTLR6, rhTIRAP, rhIL-1 RI, rhIL-1 RII, rhIL-1 RAcP, rhST2, rhIL-18 R, rhIL-1 Rrp2, rhIL-18 R beta, rhSIGIRR, rhIL-1 RAPL1, rhIL-1 RAPL2, rhMD-1, rhMD-2, or rmRP105 is observed.

Source

Monoclonal Mouse IgG_2A Clone # 610015

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TLR4
Glu24-Lys631
Accession # O00206

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of TLR4 in Human PBMC by Flow Cytometry. Human peripheral blood monocytes were stained with Human TLR4 Monoclonal Antibody (Catalog # MAB6248, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Fluorescein-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0103B).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TLR4

Toll-Like Receptor (TLR4) belongs to the IL-1 receptor/Toll-like receptor superfamily. It is expressed in placenta and peripheral blood leukocytes. TLR4 is the pattern recognition receptor for LPS.

Long Name

Toll-like Receptor 4

Entrez Gene IDs

7099 (Human); 21898 (Mouse); 29260 (Rat)

Alternate Names

ARMD10; CD_antigen: CD284; CD284 antigen; CD284; EC 3.2.2.6; EC:3.2.2.6; homolog of Drosophila toll; hToll; TLR4; TLR-4; toll like receptor 4 protein; TOLL; toll-like receptor 4